A distinct inhibitory mechanism of the V-ATPase by Vibrio VopQ revealed by cryo-EM

Wei Peng, Amanda K. Casey, Jessie Fernandez, Emily M. Carpinone, Kelly A. Servage, Zhe Chen, Yang Li, Diana R. Tomchick, Vincent J. Starai, Kim Orth

Research output: Contribution to journalArticle

Abstract

The Vibrioparahaemolyticus T3SS effector VopQ targets host-cell V-ATPase, resulting in blockage of autophagic flux and neutralization of acidic compartments. Here, we report the cryo-EM structure of VopQ bound to the Vo subcomplex of the V-ATPase. VopQ inserts into membranes and forms an unconventional pore while binding directly to subunit c of the V-ATPase membrane-embedded subcomplex Vo. We show that VopQ arrests yeast growth in vivo by targeting the immature Vo subcomplex in the endoplasmic reticulum (ER), thus providing insight into the observation that VopQ kills cells in the absence of a functional V-ATPase. VopQ is a bacterial effector that has been discovered to inhibit a host-membrane megadalton complex by coincidentally binding its target, inserting into a membrane and disrupting membrane potential. Collectively, our results reveal a mechanism by which bacterial effectors modulate host cell biology and provide an invaluable tool for future studies on V-ATPase-mediated membrane fusion and autophagy.

Original languageEnglish (US)
Pages (from-to)589-597
Number of pages9
JournalNature Structural and Molecular Biology
Volume27
Issue number6
DOIs
StatePublished - Jun 1 2020

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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