A distinct tolerogenic subset of splenic IDO+CD11b+ dendritic cells from orally tolerized mice is responsible for induction of systemic immune tolerance and suppression of collagen-induced arthritis

Min Jung Park, Kyung Su Park, Hyun Sil Park, Mi La Cho, Sue Yun Hwang, So Youn Min, Mi Kyung Park, Sung Hwan Park, Ho Youn Kim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In oral tolerance, locally instigated tolerance in the gut propagate to systemic tolerance. In order to investigate the mechanism, we analyzed indoleamine 2,3-dioxygenase (IDO) expression in splenic dendritic cell (DC) subsets and tested whether DCs suppress collagen-induced arthritis (CIA) by inducing regulatory T cells (Tregs). The proportion of IDO-expressing cells was higher in the CD11b+ subset of splenic DCs from orally tolerized CIA mice. These DCs suppressed type II collagen-specific T cell proliferation and promoted Treg induction from CD4+CD25- T cells using transforming growth factor-β. These DCs also increased the expression of cytotoxic T lymphocyte antigen-4 and programmed death-1 on Tregs. When adoptively transferred, spenic IDO-expressing CD11b+ DCs from tolerized animals suppressed the development of arthritis, increased the Treg/Th17 cell ratio, and decreased the production of inflammatory cytokines in the spleen. Taken together, a distinct subset of splenic IDO+CD11b+DCs is responsible for the systemic immune regulation in oral tolerance.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalCellular Immunology
Volume278
Issue number1-2
DOIs
StatePublished - Jul 2012

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Immune Tolerance
Experimental Arthritis
Dendritic Cells
Regulatory T-Lymphocytes
CTLA-4 Antigen
T-Lymphocytes
Th17 Cells
Collagen Type II
Transforming Growth Factors
Arthritis
Spleen
Cell Proliferation
Cytokines

Keywords

  • Collagen-induced arthritis
  • Dendritic cell
  • Indoleamine 2,3-dioxygenase
  • Oral tolerance
  • Regulatory T cell
  • Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

A distinct tolerogenic subset of splenic IDO+CD11b+ dendritic cells from orally tolerized mice is responsible for induction of systemic immune tolerance and suppression of collagen-induced arthritis. / Park, Min Jung; Park, Kyung Su; Park, Hyun Sil; Cho, Mi La; Hwang, Sue Yun; Min, So Youn; Park, Mi Kyung; Park, Sung Hwan; Kim, Ho Youn.

In: Cellular Immunology, Vol. 278, No. 1-2, 07.2012, p. 45-54.

Research output: Contribution to journalArticle

Park, Min Jung ; Park, Kyung Su ; Park, Hyun Sil ; Cho, Mi La ; Hwang, Sue Yun ; Min, So Youn ; Park, Mi Kyung ; Park, Sung Hwan ; Kim, Ho Youn. / A distinct tolerogenic subset of splenic IDO+CD11b+ dendritic cells from orally tolerized mice is responsible for induction of systemic immune tolerance and suppression of collagen-induced arthritis. In: Cellular Immunology. 2012 ; Vol. 278, No. 1-2. pp. 45-54.
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