A dramatic pH-dependent alteration in ANP receptor density

A note for using cultured cells

T. Katafuchi, H. Hagiwara, T. Ito, S. Hirose

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Culture media tend to become acidic when rapidly growing cells are cultured under batch conditions using a CO2/HCO3/- buffer system. The effects of this inherent lowering of pH on cellular makeup of cultured cells, which have long been ignored, were examined by monitoring the pH and number of the atrial natriuretic peptide (ANP) receptors expressed on the cultured bovine endothelial cells. The Eagle's minimum essential medium was adjusted to three different pH values of 7.0, 7.4, and 7.7 and used for 48-h batch cultures. After this 48-h incubation, the pH values of the media were found to be 7.0, 7.1, and 7.4, respectively. These pH shifts had unexpectedly strong influences on the ANP receptor levels without affecting the affinity. Cells maintained in the slightly higher pH medium had a trace amount of the receptor (<10 sites/cell), while those in the lower pH environment exhibited a large number of binding sites (40,000 sites/cell). Similar situations might occur in other cellular components and in other types of cells, and therefore, such possibilities should be kept in mind when cultured cell systems are used.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume264
Issue number5 33-5
StatePublished - 1993

Fingerprint

Atrial Natriuretic Factor Receptors
Cultured Cells
Cells
Endothelial cells
Culture Media
Buffers
Binding Sites
Monitoring
Eagles
Batch Cell Culture Techniques
Endothelial Cells

Keywords

  • guanosine 3',5'-cyclic monophosphate response
  • pH shift during cell culture
  • vascular endothelial cells

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

A dramatic pH-dependent alteration in ANP receptor density : A note for using cultured cells. / Katafuchi, T.; Hagiwara, H.; Ito, T.; Hirose, S.

In: American Journal of Physiology - Cell Physiology, Vol. 264, No. 5 33-5, 1993.

Research output: Contribution to journalArticle

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AU - Hirose, S.

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