A Drosophila mutant defective in extracellular calcium-dependent photoreceptor deactivation and rapid desensitization

Rama Ranganathan, Greg L. Harris, Charles F. Stevens, Charles S. Zuker

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Calcium is involved in the adaptation of vertebrate photoreceptors to light1,2 and may have a similar role in invertebrate phototransduction3,4. But the molecular mechanisms mediating this stimulus-dependent regulation are not well understood in any G protein-coupled transduction system. We have developed a preparation of isolated Drosophila photoreceptors that has allowed us to carry out an electrophysiological characterization of the light-activated response in these sensory neurons using patch-clamp techniques. We report here that extracellular calcium entering through the light-activated conductance is a key regulator of both the activation and deactivation phases of the phototransduction cascade, and that inaC mutant photoreceptors5 are specifically defective in the calcium-dependent deactivation mechanism. These data suggest that the light-dependent calcium influx inactivates this cascade through a biochemical pathway that requires the inaC gene product, and that this mechanism represents a molecular basis for stimulus-dependent regulation of visual transduction in Drosophila photoreceptors.

Original languageEnglish (US)
Pages (from-to)230-232
Number of pages3
JournalNature
Volume354
Issue number6350
StatePublished - Nov 21 1991

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Drosophila
Calcium
Light
Light Signal Transduction
Vertebrate Photoreceptor Cells
Patch-Clamp Techniques
Sensory Receptor Cells
Invertebrates
GTP-Binding Proteins
Psychologic Desensitization
Genes

ASJC Scopus subject areas

  • General

Cite this

Ranganathan, R., Harris, G. L., Stevens, C. F., & Zuker, C. S. (1991). A Drosophila mutant defective in extracellular calcium-dependent photoreceptor deactivation and rapid desensitization. Nature, 354(6350), 230-232.

A Drosophila mutant defective in extracellular calcium-dependent photoreceptor deactivation and rapid desensitization. / Ranganathan, Rama; Harris, Greg L.; Stevens, Charles F.; Zuker, Charles S.

In: Nature, Vol. 354, No. 6350, 21.11.1991, p. 230-232.

Research output: Contribution to journalArticle

Ranganathan, R, Harris, GL, Stevens, CF & Zuker, CS 1991, 'A Drosophila mutant defective in extracellular calcium-dependent photoreceptor deactivation and rapid desensitization', Nature, vol. 354, no. 6350, pp. 230-232.
Ranganathan R, Harris GL, Stevens CF, Zuker CS. A Drosophila mutant defective in extracellular calcium-dependent photoreceptor deactivation and rapid desensitization. Nature. 1991 Nov 21;354(6350):230-232.
Ranganathan, Rama ; Harris, Greg L. ; Stevens, Charles F. ; Zuker, Charles S. / A Drosophila mutant defective in extracellular calcium-dependent photoreceptor deactivation and rapid desensitization. In: Nature. 1991 ; Vol. 354, No. 6350. pp. 230-232.
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