A functional element necessary for fetal hemoglobin silencing

Vijay G. Sankaran, Jian Xu, Rachel Byron, Harvey A. Greisman, Chris Fisher, David J. Weatherall, Daniel E. Sabath, Mark Groudine, Stuart H. Orkin, Anuja Premawardhena, M. A. Bender

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

Background: An improved understanding of the regulation of the fetal hemoglobin genes holds promise for the development of targeted therapeutic approaches for fetal hemoglobin induction in the β-hemoglobinopathies. Although recent studies have uncovered trans-acting factors necessary for this regulation, limited insight has been gained into the cis-regulatory elements involved. Methods: We identified three families with unusual patterns of hemoglobin expression, suggestive of deletions in the locus of the β-globin gene (β-globin locus). We performed array comparative genomic hybridization to map these deletions and confirmed breakpoints by means of polymerase-chain-reaction assays and DNA sequencing. We compared these deletions, along with previously mapped deletions, and studied the trans-acting factors binding to these sites in the β-globin locus by using chromatin immunoprecipitation. Results: We found a new (δβ)0- thalassemia deletion and a rare hereditary persistence of fetal hemoglobin deletion with identical downstream breakpoints. Comparison of the two deletions resulted in the identification of a small intergenic region required for γ-globin (fetal hemoglobin) gene silencing. We mapped a Kurdish β0-thalassemia deletion, which retains the required intergenic region, deletes other surrounding sequences, and maintains fetal hemoglobin silencing. By comparing these deletions and other previously mapped deletions, we elucidated a 3.5-kb intergenic region near the 5′ end of the δ-globin gene that is necessary for γ-globin silencing. We found that a critical fetal hemoglobin silencing factor, BCL11A, and its partners bind within this region in the chromatin of adult erythroid cells. Conclusions: By studying three families with unusual deletions in the β-globin locus, we identified an intergenic region near the δ-globin gene that is necessary for fetal hemoglobin silencing. (Funded by the National Institutes of Health and others.)

Original languageEnglish (US)
Pages (from-to)807-814
Number of pages8
JournalNew England Journal of Medicine
Volume365
Issue number9
DOIs
Publication statusPublished - Sep 1 2011

    Fingerprint

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sankaran, V. G., Xu, J., Byron, R., Greisman, H. A., Fisher, C., Weatherall, D. J., ... Bender, M. A. (2011). A functional element necessary for fetal hemoglobin silencing. New England Journal of Medicine, 365(9), 807-814. https://doi.org/10.1056/NEJMoa1103070