A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance

Kelly V. Ruggles; Jeanne Garbarino; Ying Liu; James Moon; Kerry Schneider; Annette Henneberry; Jeff Billheimer; John S. Millar; Dawn Marchadier; Mark A. Valasek; Aidan Joblin-Mills; Sonia Gulati; Andrew B. Munkacsi; Joyce J. Repa; Dan Rader; Stephen L. Sturley

doi:10.1074/jbc.M113.515197

A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance

Kelly V. Ruggles, Jeanne Garbarino, Ying Liu, James Moon, Kerry Schneider, Annette Henneberry, Jeff Billheimer, John S. Millar, Dawn Marchadier, Mark A. Valasek, Aidan Joblin-Mills, Sonia Gulati, Andrew B. Munkacsi, Joyce J. Repa, Dan Rader, Stephen L. Sturley

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Background: Obesity-related diseases result from accumulation of lipids in nonadipose tissues. Results: Mutations in 167 yeast genes confer fatty acid sensitivity. Loss of yeast and mammalian ARV1 results in pronounced lipid hypersensitivity, lipoapoptosis, and reduced triglyceride synthesis. Conclusion: 75 evolutionarily conserved components of obesity-related disorders were identified. Significance: Understanding lipid sensitivity may lead to treatment of numerous human metabolic diseases.

Original language	English (US)
Pages (from-to)	4417-4431
Number of pages	15
Journal	Journal of Biological Chemistry
Volume	289
Issue number	7
DOIs	https://doi.org/10.1074/jbc.M113.515197
State	Published - Feb 14 2014

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.M113.515197

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Ruggles, K. V., Garbarino, J., Liu, Y., Moon, J., Schneider, K., Henneberry, A., Billheimer, J., Millar, J. S., Marchadier, D., Valasek, M. A., Joblin-Mills, A., Gulati, S., Munkacsi, A. B., Repa, J. J., Rader, D., & Sturley, S. L. (2014). A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance. Journal of Biological Chemistry, 289(7), 4417-4431. https://doi.org/10.1074/jbc.M113.515197

A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance. / Ruggles, Kelly V.; Garbarino, Jeanne; Liu, Ying et al.
In: Journal of Biological Chemistry, Vol. 289, No. 7, 14.02.2014, p. 4417-4431.

Research output: Contribution to journal › Article › peer-review

Ruggles, KV, Garbarino, J, Liu, Y, Moon, J, Schneider, K, Henneberry, A, Billheimer, J, Millar, JS, Marchadier, D, Valasek, MA, Joblin-Mills, A, Gulati, S, Munkacsi, AB, Repa, JJ, Rader, D & Sturley, SL 2014, 'A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance', Journal of Biological Chemistry, vol. 289, no. 7, pp. 4417-4431. https://doi.org/10.1074/jbc.M113.515197

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abstract = "Background: Obesity-related diseases result from accumulation of lipids in nonadipose tissues. Results: Mutations in 167 yeast genes confer fatty acid sensitivity. Loss of yeast and mammalian ARV1 results in pronounced lipid hypersensitivity, lipoapoptosis, and reduced triglyceride synthesis. Conclusion: 75 evolutionarily conserved components of obesity-related disorders were identified. Significance: Understanding lipid sensitivity may lead to treatment of numerous human metabolic diseases.",

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AU - Moon, James

AU - Schneider, Kerry

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AU - Billheimer, Jeff

AU - Millar, John S.

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AU - Valasek, Mark A.

AU - Joblin-Mills, Aidan

AU - Gulati, Sonia

AU - Munkacsi, Andrew B.

AU - Repa, Joyce J.

AU - Rader, Dan

AU - Sturley, Stephen L.

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A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance

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