A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance

Kelly V. Ruggles, Jeanne Garbarino, Ying Liu, James Moon, Kerry Schneider, Annette Henneberry, Jeff Billheimer, John S. Millar, Dawn Marchadier, Mark A. Valasek, Aidan Joblin-Mills, Sonia Gulati, Andrew B. Munkacsi, Joyce J. Repa, Dan Rader, Stephen L. Sturley

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Background: Obesity-related diseases result from accumulation of lipids in nonadipose tissues. Results: Mutations in 167 yeast genes confer fatty acid sensitivity. Loss of yeast and mammalian ARV1 results in pronounced lipid hypersensitivity, lipoapoptosis, and reduced triglyceride synthesis. Conclusion: 75 evolutionarily conserved components of obesity-related disorders were identified. Significance: Understanding lipid sensitivity may lead to treatment of numerous human metabolic diseases.

Original languageEnglish (US)
Pages (from-to)4417-4431
Number of pages15
JournalJournal of Biological Chemistry
Volume289
Issue number7
DOIs
StatePublished - Feb 14 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Ruggles, K. V., Garbarino, J., Liu, Y., Moon, J., Schneider, K., Henneberry, A., Billheimer, J., Millar, J. S., Marchadier, D., Valasek, M. A., Joblin-Mills, A., Gulati, S., Munkacsi, A. B., Repa, J. J., Rader, D., & Sturley, S. L. (2014). A functional, genome-wide evaluation of liposensitive yeast identifies the ARE2 required for viability (ARV1) gene product as a major component of eukaryotic fatty acid resistance. Journal of Biological Chemistry, 289(7), 4417-4431. https://doi.org/10.1074/jbc.M113.515197