A functional retinoic acid receptor encoded by the gene on human chromosome 12

Takashi Ishikawa, Kazuhiko Umesono, David J. Mangelsdorf, Hiroyuki Aburatani, Ben Z. Stanger, Yoshikazu Shibasaki, Michio Imawari, Ronald M. Evans, Fumimaro Takaku

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

A cDNA clone derived from a human hepatocellular carcinoma has been isolated on the basis of homology to the a human retinoic acid receptor (RARa) gene. Expression of this cDNA produces a high affinity nuclear binding protein for retinoic acid. The product of this clone when expressed in transfected cells is able to activate transcription of a reporter plasmid through specific DNA sequences in response to the addition of retinoic acid to the medium. Dose-dependent profiles upon trans-activation of the reporter indicate that apparent sensitivity to retinoic acid of this protein is approximately 10-fold higher than that of human RARα and is comparable to that of the second human RAR, RARβ. This gene has been mapped to human chromosome 12, which is distinct from those coding for either α or β RAR, and thus encodes a third human RAR.

Original languageEnglish (US)
Pages (from-to)837-844
Number of pages8
JournalMolecular Endocrinology
Volume4
Issue number6
StatePublished - Jun 1990

Fingerprint

Chromosomes, Human, Pair 12
Retinoic Acid Receptors
Human Chromosomes
Tretinoin
Genes
Complementary DNA
Clone Cells
Nuclear Proteins
Hepatocellular Carcinoma
Carrier Proteins
Plasmids
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ishikawa, T., Umesono, K., Mangelsdorf, D. J., Aburatani, H., Stanger, B. Z., Shibasaki, Y., ... Takaku, F. (1990). A functional retinoic acid receptor encoded by the gene on human chromosome 12. Molecular Endocrinology, 4(6), 837-844.

A functional retinoic acid receptor encoded by the gene on human chromosome 12. / Ishikawa, Takashi; Umesono, Kazuhiko; Mangelsdorf, David J.; Aburatani, Hiroyuki; Stanger, Ben Z.; Shibasaki, Yoshikazu; Imawari, Michio; Evans, Ronald M.; Takaku, Fumimaro.

In: Molecular Endocrinology, Vol. 4, No. 6, 06.1990, p. 837-844.

Research output: Contribution to journalArticle

Ishikawa, T, Umesono, K, Mangelsdorf, DJ, Aburatani, H, Stanger, BZ, Shibasaki, Y, Imawari, M, Evans, RM & Takaku, F 1990, 'A functional retinoic acid receptor encoded by the gene on human chromosome 12', Molecular Endocrinology, vol. 4, no. 6, pp. 837-844.
Ishikawa T, Umesono K, Mangelsdorf DJ, Aburatani H, Stanger BZ, Shibasaki Y et al. A functional retinoic acid receptor encoded by the gene on human chromosome 12. Molecular Endocrinology. 1990 Jun;4(6):837-844.
Ishikawa, Takashi ; Umesono, Kazuhiko ; Mangelsdorf, David J. ; Aburatani, Hiroyuki ; Stanger, Ben Z. ; Shibasaki, Yoshikazu ; Imawari, Michio ; Evans, Ronald M. ; Takaku, Fumimaro. / A functional retinoic acid receptor encoded by the gene on human chromosome 12. In: Molecular Endocrinology. 1990 ; Vol. 4, No. 6. pp. 837-844.
@article{5c7cd99cba3e43928ae91432d090edd7,
title = "A functional retinoic acid receptor encoded by the gene on human chromosome 12",
abstract = "A cDNA clone derived from a human hepatocellular carcinoma has been isolated on the basis of homology to the a human retinoic acid receptor (RARa) gene. Expression of this cDNA produces a high affinity nuclear binding protein for retinoic acid. The product of this clone when expressed in transfected cells is able to activate transcription of a reporter plasmid through specific DNA sequences in response to the addition of retinoic acid to the medium. Dose-dependent profiles upon trans-activation of the reporter indicate that apparent sensitivity to retinoic acid of this protein is approximately 10-fold higher than that of human RARα and is comparable to that of the second human RAR, RARβ. This gene has been mapped to human chromosome 12, which is distinct from those coding for either α or β RAR, and thus encodes a third human RAR.",
author = "Takashi Ishikawa and Kazuhiko Umesono and Mangelsdorf, {David J.} and Hiroyuki Aburatani and Stanger, {Ben Z.} and Yoshikazu Shibasaki and Michio Imawari and Evans, {Ronald M.} and Fumimaro Takaku",
year = "1990",
month = "6",
language = "English (US)",
volume = "4",
pages = "837--844",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - A functional retinoic acid receptor encoded by the gene on human chromosome 12

AU - Ishikawa, Takashi

AU - Umesono, Kazuhiko

AU - Mangelsdorf, David J.

AU - Aburatani, Hiroyuki

AU - Stanger, Ben Z.

AU - Shibasaki, Yoshikazu

AU - Imawari, Michio

AU - Evans, Ronald M.

AU - Takaku, Fumimaro

PY - 1990/6

Y1 - 1990/6

N2 - A cDNA clone derived from a human hepatocellular carcinoma has been isolated on the basis of homology to the a human retinoic acid receptor (RARa) gene. Expression of this cDNA produces a high affinity nuclear binding protein for retinoic acid. The product of this clone when expressed in transfected cells is able to activate transcription of a reporter plasmid through specific DNA sequences in response to the addition of retinoic acid to the medium. Dose-dependent profiles upon trans-activation of the reporter indicate that apparent sensitivity to retinoic acid of this protein is approximately 10-fold higher than that of human RARα and is comparable to that of the second human RAR, RARβ. This gene has been mapped to human chromosome 12, which is distinct from those coding for either α or β RAR, and thus encodes a third human RAR.

AB - A cDNA clone derived from a human hepatocellular carcinoma has been isolated on the basis of homology to the a human retinoic acid receptor (RARa) gene. Expression of this cDNA produces a high affinity nuclear binding protein for retinoic acid. The product of this clone when expressed in transfected cells is able to activate transcription of a reporter plasmid through specific DNA sequences in response to the addition of retinoic acid to the medium. Dose-dependent profiles upon trans-activation of the reporter indicate that apparent sensitivity to retinoic acid of this protein is approximately 10-fold higher than that of human RARα and is comparable to that of the second human RAR, RARβ. This gene has been mapped to human chromosome 12, which is distinct from those coding for either α or β RAR, and thus encodes a third human RAR.

UR - http://www.scopus.com/inward/record.url?scp=0025175755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025175755&partnerID=8YFLogxK

M3 - Article

C2 - 2172793

AN - SCOPUS:0025175755

VL - 4

SP - 837

EP - 844

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 6

ER -