A gamete-specific, sex-limited homeodomain protein in Chlamydomonas

Venkatesh Kurvari, Nick V. Grishin, William J. Snell

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation for cell-cell fusion. We have identified a cDNA, gsp1, whose transcript levels are upregulated during flagellar adhesion. The GSP1 polypeptide is a novel, gamete-specific homeodomain protein, the first to be identified in an alga. Its homeodomain shows significant identity with several higher plant homeodomain proteins. Although encoded by a single copy gene present in cells of both mating types, immunoblot analysis showed that GSP1 was expressed in mating type (mt)+ gametes, but was not detectable in mt- gametes or in vegetative cells of either mating type. Moreover, GSP1 appeared late during gametogenesis, suggesting that it may function during adhesion with mt- gametes or after zygote formation. GSP1 is expressed in imp11, mt- mutant gametes, which have a lesion in the mid gene involved in sex determination and exhibit many phenotypic characteristics of mt+ gametes. Thus, gsp1 is negatively regulated by mid and is the first molecule to be identified in Chlamydomonas that shows sex-limited expression.

Original languageEnglish (US)
Pages (from-to)1971-1980
Number of pages10
JournalJournal of Cell Biology
Volume143
Issue number7
DOIs
StatePublished - Dec 28 1998

Fingerprint

Chlamydomonas
Homeodomain Proteins
Germ Cells
Gametogenesis
Plant Proteins
Cell Fusion
Zygote
Fertilization
Genes
Signal Transduction
Complementary DNA
Peptides

Keywords

  • Cell-cell adhesion
  • Chlamydomonas
  • Fertilization
  • Homeodomain
  • Sex-limited

ASJC Scopus subject areas

  • Cell Biology

Cite this

A gamete-specific, sex-limited homeodomain protein in Chlamydomonas. / Kurvari, Venkatesh; Grishin, Nick V.; Snell, William J.

In: Journal of Cell Biology, Vol. 143, No. 7, 28.12.1998, p. 1971-1980.

Research output: Contribution to journalArticle

@article{544c4fee748c413081842b6217b4e607,
title = "A gamete-specific, sex-limited homeodomain protein in Chlamydomonas",
abstract = "During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation for cell-cell fusion. We have identified a cDNA, gsp1, whose transcript levels are upregulated during flagellar adhesion. The GSP1 polypeptide is a novel, gamete-specific homeodomain protein, the first to be identified in an alga. Its homeodomain shows significant identity with several higher plant homeodomain proteins. Although encoded by a single copy gene present in cells of both mating types, immunoblot analysis showed that GSP1 was expressed in mating type (mt)+ gametes, but was not detectable in mt- gametes or in vegetative cells of either mating type. Moreover, GSP1 appeared late during gametogenesis, suggesting that it may function during adhesion with mt- gametes or after zygote formation. GSP1 is expressed in imp11, mt- mutant gametes, which have a lesion in the mid gene involved in sex determination and exhibit many phenotypic characteristics of mt+ gametes. Thus, gsp1 is negatively regulated by mid and is the first molecule to be identified in Chlamydomonas that shows sex-limited expression.",
keywords = "Cell-cell adhesion, Chlamydomonas, Fertilization, Homeodomain, Sex-limited",
author = "Venkatesh Kurvari and Grishin, {Nick V.} and Snell, {William J.}",
year = "1998",
month = "12",
day = "28",
doi = "10.1083/jcb.143.7.1971",
language = "English (US)",
volume = "143",
pages = "1971--1980",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "7",

}

TY - JOUR

T1 - A gamete-specific, sex-limited homeodomain protein in Chlamydomonas

AU - Kurvari, Venkatesh

AU - Grishin, Nick V.

AU - Snell, William J.

PY - 1998/12/28

Y1 - 1998/12/28

N2 - During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation for cell-cell fusion. We have identified a cDNA, gsp1, whose transcript levels are upregulated during flagellar adhesion. The GSP1 polypeptide is a novel, gamete-specific homeodomain protein, the first to be identified in an alga. Its homeodomain shows significant identity with several higher plant homeodomain proteins. Although encoded by a single copy gene present in cells of both mating types, immunoblot analysis showed that GSP1 was expressed in mating type (mt)+ gametes, but was not detectable in mt- gametes or in vegetative cells of either mating type. Moreover, GSP1 appeared late during gametogenesis, suggesting that it may function during adhesion with mt- gametes or after zygote formation. GSP1 is expressed in imp11, mt- mutant gametes, which have a lesion in the mid gene involved in sex determination and exhibit many phenotypic characteristics of mt+ gametes. Thus, gsp1 is negatively regulated by mid and is the first molecule to be identified in Chlamydomonas that shows sex-limited expression.

AB - During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation for cell-cell fusion. We have identified a cDNA, gsp1, whose transcript levels are upregulated during flagellar adhesion. The GSP1 polypeptide is a novel, gamete-specific homeodomain protein, the first to be identified in an alga. Its homeodomain shows significant identity with several higher plant homeodomain proteins. Although encoded by a single copy gene present in cells of both mating types, immunoblot analysis showed that GSP1 was expressed in mating type (mt)+ gametes, but was not detectable in mt- gametes or in vegetative cells of either mating type. Moreover, GSP1 appeared late during gametogenesis, suggesting that it may function during adhesion with mt- gametes or after zygote formation. GSP1 is expressed in imp11, mt- mutant gametes, which have a lesion in the mid gene involved in sex determination and exhibit many phenotypic characteristics of mt+ gametes. Thus, gsp1 is negatively regulated by mid and is the first molecule to be identified in Chlamydomonas that shows sex-limited expression.

KW - Cell-cell adhesion

KW - Chlamydomonas

KW - Fertilization

KW - Homeodomain

KW - Sex-limited

UR - http://www.scopus.com/inward/record.url?scp=0032576582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032576582&partnerID=8YFLogxK

U2 - 10.1083/jcb.143.7.1971

DO - 10.1083/jcb.143.7.1971

M3 - Article

VL - 143

SP - 1971

EP - 1980

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 7

ER -