A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA

H. H. Shu, C. A. Wise, G. D. Clark-Walker, N. C. Martin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have mapped a gene in the mitochondrial DNA of Candida (Torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial tRNAs. It is located between the tRNA(f)(Met) and tRNA(Pro) genes, the same tRNA genes that flank the mitochondrial RNase P RNA gene in the yeast Saccharomyces cerevisiae. The gene is extremely AT rich and codes for AU-rich RNAs that display some sequence homology with the mitochondrial RNase P RNA from S. cerevisiae, including two regions of striking sequence homology between the mitochondrial RNAs and the bacterial RNase P . RNase P activity that is sensitive to micrococcal nuclease has been detected in mitochondrial extracts of C. glabrata. An RNA of 227 nucleotides that is one of the RNAs encoded by the gene that we mapped cofractionated with this mitochondrial RNase P activity on glycerol gradients. The nuclease sensitivity of the activity, the cofractionation of the RNA with activity, and the homology of the RNA with known RNase P RNAs lead us to propose that the 227-nucleotide RNA is the RNA subunit of the C. glabrata mitochondrial RNase P enzyme.

Original languageEnglish (US)
Pages (from-to)1662-1667
Number of pages6
JournalMolecular and Cellular Biology
Volume11
Issue number3
DOIs
StatePublished - Jan 1 1991

Fingerprint

Ribonuclease P
Candida glabrata
Mitochondria
Nucleotides
RNA
Genes
Sequence Homology
Transfer RNA
Saccharomyces cerevisiae
RNA, Transfer, Pro
RNA, Transfer, Met
Micrococcal Nuclease
Mitochondrial Genes
Mitochondrial DNA
Glycerol
Yeasts

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA. / Shu, H. H.; Wise, C. A.; Clark-Walker, G. D.; Martin, N. C.

In: Molecular and Cellular Biology, Vol. 11, No. 3, 01.01.1991, p. 1662-1667.

Research output: Contribution to journalArticle

@article{c6afa93325434ae0b1b22914ebbcd132,
title = "A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA",
abstract = "We have mapped a gene in the mitochondrial DNA of Candida (Torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial tRNAs. It is located between the tRNA(f)(Met) and tRNA(Pro) genes, the same tRNA genes that flank the mitochondrial RNase P RNA gene in the yeast Saccharomyces cerevisiae. The gene is extremely AT rich and codes for AU-rich RNAs that display some sequence homology with the mitochondrial RNase P RNA from S. cerevisiae, including two regions of striking sequence homology between the mitochondrial RNAs and the bacterial RNase P . RNase P activity that is sensitive to micrococcal nuclease has been detected in mitochondrial extracts of C. glabrata. An RNA of 227 nucleotides that is one of the RNAs encoded by the gene that we mapped cofractionated with this mitochondrial RNase P activity on glycerol gradients. The nuclease sensitivity of the activity, the cofractionation of the RNA with activity, and the homology of the RNA with known RNase P RNAs lead us to propose that the 227-nucleotide RNA is the RNA subunit of the C. glabrata mitochondrial RNase P enzyme.",
author = "Shu, {H. H.} and Wise, {C. A.} and Clark-Walker, {G. D.} and Martin, {N. C.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1128/MCB.11.3.1662",
language = "English (US)",
volume = "11",
pages = "1662--1667",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - A gene required for RNase P activity in Candida (Torulopsis) glabrata mitochondria codes for a 227-nucleotide RNA with homology to bacterial RNase P RNA

AU - Shu, H. H.

AU - Wise, C. A.

AU - Clark-Walker, G. D.

AU - Martin, N. C.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - We have mapped a gene in the mitochondrial DNA of Candida (Torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial tRNAs. It is located between the tRNA(f)(Met) and tRNA(Pro) genes, the same tRNA genes that flank the mitochondrial RNase P RNA gene in the yeast Saccharomyces cerevisiae. The gene is extremely AT rich and codes for AU-rich RNAs that display some sequence homology with the mitochondrial RNase P RNA from S. cerevisiae, including two regions of striking sequence homology between the mitochondrial RNAs and the bacterial RNase P . RNase P activity that is sensitive to micrococcal nuclease has been detected in mitochondrial extracts of C. glabrata. An RNA of 227 nucleotides that is one of the RNAs encoded by the gene that we mapped cofractionated with this mitochondrial RNase P activity on glycerol gradients. The nuclease sensitivity of the activity, the cofractionation of the RNA with activity, and the homology of the RNA with known RNase P RNAs lead us to propose that the 227-nucleotide RNA is the RNA subunit of the C. glabrata mitochondrial RNase P enzyme.

AB - We have mapped a gene in the mitochondrial DNA of Candida (Torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial tRNAs. It is located between the tRNA(f)(Met) and tRNA(Pro) genes, the same tRNA genes that flank the mitochondrial RNase P RNA gene in the yeast Saccharomyces cerevisiae. The gene is extremely AT rich and codes for AU-rich RNAs that display some sequence homology with the mitochondrial RNase P RNA from S. cerevisiae, including two regions of striking sequence homology between the mitochondrial RNAs and the bacterial RNase P . RNase P activity that is sensitive to micrococcal nuclease has been detected in mitochondrial extracts of C. glabrata. An RNA of 227 nucleotides that is one of the RNAs encoded by the gene that we mapped cofractionated with this mitochondrial RNase P activity on glycerol gradients. The nuclease sensitivity of the activity, the cofractionation of the RNA with activity, and the homology of the RNA with known RNase P RNAs lead us to propose that the 227-nucleotide RNA is the RNA subunit of the C. glabrata mitochondrial RNase P enzyme.

UR - http://www.scopus.com/inward/record.url?scp=0026013147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026013147&partnerID=8YFLogxK

U2 - 10.1128/MCB.11.3.1662

DO - 10.1128/MCB.11.3.1662

M3 - Article

VL - 11

SP - 1662

EP - 1667

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 3

ER -