A genetic approach to promoter recognition during trans induction of viral gene expression

Donald M. Coen, Steven P. Weinheimer, Steven L. Mcknight

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalScience
Volume234
Issue number4772
StatePublished - 1986

Fingerprint

Viral Genes
Thymidine Kinase
Viral Regulatory and Accessory Proteins
Gene Expression
Transcription Factors
Simplexvirus
Mutation
Gene Expression Regulation
Virus Diseases
Chromosomes
Genes

ASJC Scopus subject areas

  • General

Cite this

A genetic approach to promoter recognition during trans induction of viral gene expression. / Coen, Donald M.; Weinheimer, Steven P.; Mcknight, Steven L.

In: Science, Vol. 234, No. 4772, 1986, p. 53-59.

Research output: Contribution to journalArticle

Coen, Donald M. ; Weinheimer, Steven P. ; Mcknight, Steven L. / A genetic approach to promoter recognition during trans induction of viral gene expression. In: Science. 1986 ; Vol. 234, No. 4772. pp. 53-59.
@article{9891149854c14121aa55ef4274f0bedd,
title = "A genetic approach to promoter recognition during trans induction of viral gene expression",
abstract = "Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.",
author = "Coen, {Donald M.} and Weinheimer, {Steven P.} and Mcknight, {Steven L.}",
year = "1986",
language = "English (US)",
volume = "234",
pages = "53--59",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4772",

}

TY - JOUR

T1 - A genetic approach to promoter recognition during trans induction of viral gene expression

AU - Coen, Donald M.

AU - Weinheimer, Steven P.

AU - Mcknight, Steven L.

PY - 1986

Y1 - 1986

N2 - Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.

AB - Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.

UR - http://www.scopus.com/inward/record.url?scp=0022995745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022995745&partnerID=8YFLogxK

M3 - Article

C2 - 3018926

AN - SCOPUS:0022995745

VL - 234

SP - 53

EP - 59

JO - Science

JF - Science

SN - 0036-8075

IS - 4772

ER -