A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis

Mary Anne Pultz, Kristin K. Zimmerman, Neal M. Alto, Matt Kaeberlein, Sarah K. Lange, Jason N. Pitt, Nick L. Reeves, Darin L. Zehrung

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have screened for zygotic embryonic lethal mutations affecting cuticular morphology in Nasonia vitripennis (Hymenoptera; Chalcidoidea). Our broad goal was to investigate the use of Nasonia for genetically surveying conservation and change in regulatory gene systems, as a means to understand the diversity of developmental strategies that have arisen during the course of evolution. Specifically, we aim to compare anteroposterior patterning gene functions in two long germ band insects, Nasonia and Drosophila. In Nasonia, unfertilized eggs develop as haploid males while fertilized eggs develop as diploid females, so the entire genome can be screened for recessive zygotic mutations by examining the progeny of F1 females. We describe 74 of >100 lines with embryonic cuticular mutant phenotypes, including representatives of coordinate, gap, pair-rule, segment polarity, homeotic, and Polycomb group functions, as well as mutants with novel phenotypes not directly comparable to those of known Drosophila genes. We conclude that Nasonia is a tractable experimental organism for comparative developmental genetic study. The mutants isolated here have begun to outline the extent of conservation and change in the genetic programs controlling embryonic patterning in Nasonia and Drosophila.

Original languageEnglish (US)
Pages (from-to)1213-1229
Number of pages17
JournalGenetics
Volume154
Issue number3
StatePublished - Mar 2000

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Wasps
Drosophila
Mutation
Phenotype
Hymenoptera
Zygote
Haploidy
Regulator Genes
Diploidy
Genes
Ovum
Insects
Genome

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Pultz, M. A., Zimmerman, K. K., Alto, N. M., Kaeberlein, M., Lange, S. K., Pitt, J. N., ... Zehrung, D. L. (2000). A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis. Genetics, 154(3), 1213-1229.

A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis. / Pultz, Mary Anne; Zimmerman, Kristin K.; Alto, Neal M.; Kaeberlein, Matt; Lange, Sarah K.; Pitt, Jason N.; Reeves, Nick L.; Zehrung, Darin L.

In: Genetics, Vol. 154, No. 3, 03.2000, p. 1213-1229.

Research output: Contribution to journalArticle

Pultz, MA, Zimmerman, KK, Alto, NM, Kaeberlein, M, Lange, SK, Pitt, JN, Reeves, NL & Zehrung, DL 2000, 'A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis', Genetics, vol. 154, no. 3, pp. 1213-1229.
Pultz MA, Zimmerman KK, Alto NM, Kaeberlein M, Lange SK, Pitt JN et al. A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis. Genetics. 2000 Mar;154(3):1213-1229.
Pultz, Mary Anne ; Zimmerman, Kristin K. ; Alto, Neal M. ; Kaeberlein, Matt ; Lange, Sarah K. ; Pitt, Jason N. ; Reeves, Nick L. ; Zehrung, Darin L. / A genetic screen for zygotic embryonic lethal mutations affecting cuticular morphology in the wasp Nasonia vitripennis. In: Genetics. 2000 ; Vol. 154, No. 3. pp. 1213-1229.
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