A genomewide screen for components of the RNAi pathway in Drosophila cultured cells

Silke Dorner, Lawrence Lum, Michelle Kim, Renato Paro, Philip A. Beachy, Rachel Green

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).

Original languageEnglish (US)
Pages (from-to)11880-11885
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number32
DOIs
StatePublished - Aug 8 2006

Fingerprint

RNA Interference
Drosophila
Cultured Cells
RNA Stability
Drosophila melanogaster
Luciferases
Genes

Keywords

  • dsRNA
  • Posttranscriptional silencing
  • RNAi screen
  • siRNA
  • siRNA pathway

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A genomewide screen for components of the RNAi pathway in Drosophila cultured cells. / Dorner, Silke; Lum, Lawrence; Kim, Michelle; Paro, Renato; Beachy, Philip A.; Green, Rachel.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 32, 08.08.2006, p. 11880-11885.

Research output: Contribution to journalArticle

Dorner, Silke ; Lum, Lawrence ; Kim, Michelle ; Paro, Renato ; Beachy, Philip A. ; Green, Rachel. / A genomewide screen for components of the RNAi pathway in Drosophila cultured cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 32. pp. 11880-11885.
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