A hexanucleotide element directs microRNA nuclear import

Hun Way Hwang, Erik A. Wentzel, Joshua T. Mendell

Research output: Contribution to journalArticle

452 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) negatively regulate partially complementary target messenger RNAs. Target selection in animals is dictated primarily by sequences at the miRNA 5′ end. We demonstrated that despite their small size, specific miRNAs contain additional sequence elements that control their posttranscriptional behavior, including their subcellular localization. We showed that human miR-29b, in contrast to other studied animal miRNAs, is predominantly localized to the nucleus. The distinctive hexanucleotide terminal motif of miR-29b acts as a transferable nuclear localization element that directs nuclear enrichment of miRNAs or small interfering RNAs to which it is attached. Our results indicate that miRNAs sharing common 5′ sequences, considered to be largely redundant, might have distinct functions because of the influence of cis-acting regulatory motifs.

Original languageEnglish (US)
Pages (from-to)97-100
Number of pages4
JournalScience
Volume315
Issue number5808
DOIs
StatePublished - Jan 5 2007

Fingerprint

Cell Nucleus Active Transport
MicroRNAs
Small Interfering RNA
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

A hexanucleotide element directs microRNA nuclear import. / Hwang, Hun Way; Wentzel, Erik A.; Mendell, Joshua T.

In: Science, Vol. 315, No. 5808, 05.01.2007, p. 97-100.

Research output: Contribution to journalArticle

Hwang, Hun Way ; Wentzel, Erik A. ; Mendell, Joshua T. / A hexanucleotide element directs microRNA nuclear import. In: Science. 2007 ; Vol. 315, No. 5808. pp. 97-100.
@article{b13887fca8064c5c973341ee0c1a2fc5,
title = "A hexanucleotide element directs microRNA nuclear import",
abstract = "MicroRNAs (miRNAs) negatively regulate partially complementary target messenger RNAs. Target selection in animals is dictated primarily by sequences at the miRNA 5′ end. We demonstrated that despite their small size, specific miRNAs contain additional sequence elements that control their posttranscriptional behavior, including their subcellular localization. We showed that human miR-29b, in contrast to other studied animal miRNAs, is predominantly localized to the nucleus. The distinctive hexanucleotide terminal motif of miR-29b acts as a transferable nuclear localization element that directs nuclear enrichment of miRNAs or small interfering RNAs to which it is attached. Our results indicate that miRNAs sharing common 5′ sequences, considered to be largely redundant, might have distinct functions because of the influence of cis-acting regulatory motifs.",
author = "Hwang, {Hun Way} and Wentzel, {Erik A.} and Mendell, {Joshua T.}",
year = "2007",
month = "1",
day = "5",
doi = "10.1126/science.1136235",
language = "English (US)",
volume = "315",
pages = "97--100",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5808",

}

TY - JOUR

T1 - A hexanucleotide element directs microRNA nuclear import

AU - Hwang, Hun Way

AU - Wentzel, Erik A.

AU - Mendell, Joshua T.

PY - 2007/1/5

Y1 - 2007/1/5

N2 - MicroRNAs (miRNAs) negatively regulate partially complementary target messenger RNAs. Target selection in animals is dictated primarily by sequences at the miRNA 5′ end. We demonstrated that despite their small size, specific miRNAs contain additional sequence elements that control their posttranscriptional behavior, including their subcellular localization. We showed that human miR-29b, in contrast to other studied animal miRNAs, is predominantly localized to the nucleus. The distinctive hexanucleotide terminal motif of miR-29b acts as a transferable nuclear localization element that directs nuclear enrichment of miRNAs or small interfering RNAs to which it is attached. Our results indicate that miRNAs sharing common 5′ sequences, considered to be largely redundant, might have distinct functions because of the influence of cis-acting regulatory motifs.

AB - MicroRNAs (miRNAs) negatively regulate partially complementary target messenger RNAs. Target selection in animals is dictated primarily by sequences at the miRNA 5′ end. We demonstrated that despite their small size, specific miRNAs contain additional sequence elements that control their posttranscriptional behavior, including their subcellular localization. We showed that human miR-29b, in contrast to other studied animal miRNAs, is predominantly localized to the nucleus. The distinctive hexanucleotide terminal motif of miR-29b acts as a transferable nuclear localization element that directs nuclear enrichment of miRNAs or small interfering RNAs to which it is attached. Our results indicate that miRNAs sharing common 5′ sequences, considered to be largely redundant, might have distinct functions because of the influence of cis-acting regulatory motifs.

UR - http://www.scopus.com/inward/record.url?scp=33846699083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846699083&partnerID=8YFLogxK

U2 - 10.1126/science.1136235

DO - 10.1126/science.1136235

M3 - Article

C2 - 17204650

AN - SCOPUS:33846699083

VL - 315

SP - 97

EP - 100

JO - Science

JF - Science

SN - 0036-8075

IS - 5808

ER -