A high-resolution morphological and ultrastructural map of anterior sensory cilia and glia in Caenorhabditis elegans

David B. Doroquez, Cristina Berciu, James R. Anderson, Piali Sengupta, Daniela Nicastro

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Many primary sensory cilia exhibit unique architectures that are critical for transduction of specific sensory stimuli. Although basic ciliogenic mechanisms are well described, how complex ciliary structures are generated remains unclear. Seminal work performed several decades ago provided an initial but incomplete description of diverse sensory cilia morphologies in C. elegans. To begin to explore the mechanisms that generate these remarkably complex structures, we have taken advantage of advances in electron microscopy and tomography, and reconstructed three-dimensional structures of fifty of sixty sensory cilia in the C. elegans adult hermaphrodite at high resolution. We characterize novel axonemal microtubule organization patterns, clarify structural features at the ciliary base, describe new aspects of cilia-glia interactions, and identify structures suggesting novel mechanisms of ciliary protein trafficking. This complete ultrastructural description of diverse cilia in C. elegans provides the foundation for investigations into underlying ciliogenic pathways, as well as contributions of defined ciliary structures to specific neuronal functions.

Original languageEnglish (US)
Article numbere01948
JournaleLife
Volume2014
Issue number3
DOIs
StatePublished - Mar 25 2014

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Cilia
Caenorhabditis elegans
Neuroglia
Electron microscopy
Tomography
Proteins
Electron Microscope Tomography
Protein Transport
Microtubules
Electron Microscopy

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A high-resolution morphological and ultrastructural map of anterior sensory cilia and glia in Caenorhabditis elegans. / Doroquez, David B.; Berciu, Cristina; Anderson, James R.; Sengupta, Piali; Nicastro, Daniela.

In: eLife, Vol. 2014, No. 3, e01948, 25.03.2014.

Research output: Contribution to journalArticle

Doroquez, David B. ; Berciu, Cristina ; Anderson, James R. ; Sengupta, Piali ; Nicastro, Daniela. / A high-resolution morphological and ultrastructural map of anterior sensory cilia and glia in Caenorhabditis elegans. In: eLife. 2014 ; Vol. 2014, No. 3.
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