A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: Testosterone production by dispersed leydig cells

M. L. Dufau, C. R. Mendelson, K. J. Catt

Research output: Contribution to journalArticle

214 Scopus citations

Abstract

The in vitro bioassay for LH/hCG employing isolated rat testes has been significantly improved by utilization of collagenase-dispersed interstitial cells in the presence of phosphodiesterase inhibitors. The sensitivity of the assay system has been extended to 20µIU (2 picograms) of hCG, and the index of precision of the method (λ) is less than 0.04. The high sensitivity of the interstitial cell assay permits bioassay of circulating gonadotropins in human plasma.

Original languageEnglish (US)
Pages (from-to)610-613
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume39
Issue number3
DOIs
StatePublished - Sep 1974

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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