A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: testosterone production by disperse Leydig cells

M. L. Dufau, C. R. Mendelson, K. J. Catt

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

The in vitro bioassay for LH/hCG employing isolated rat testes has been significantly improved by utilization of collagenase dispersed interstitial cells in the presence of phosphodiesterase inhibitors. The sensitivity of the assay system has been extended to 20 μIU (2 picograms) of hCG, and the index of precision of the method is less than 0.04. The high sensitivity of the interstitial cell assay permits bioassay of circulating gonadotropins in human plasma.

Original languageEnglish (US)
Pages (from-to)610-613
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume39
Issue number3
StatePublished - 1974

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Leydig Cells
Bioassay
Chorionic Gonadotropin
Luteinizing Hormone
Biological Assay
Testosterone
Assays
Plasma (human)
Matrix Metalloproteinase 1
Phosphodiesterase Inhibitors
Collagenases
Gonadotropins
Testis
Rats
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

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abstract = "The in vitro bioassay for LH/hCG employing isolated rat testes has been significantly improved by utilization of collagenase dispersed interstitial cells in the presence of phosphodiesterase inhibitors. The sensitivity of the assay system has been extended to 20 μIU (2 picograms) of hCG, and the index of precision of the method is less than 0.04. The high sensitivity of the interstitial cell assay permits bioassay of circulating gonadotropins in human plasma.",
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