A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: Testosterone production by dispersed leydig cells

M. L. Dufau; C. R. Mendelson; K. J. Catt

doi:10.1210/jcem-39-3-610

A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: Testosterone production by dispersed leydig cells

M. L. Dufau, C. R. Mendelson, K. J. Catt

Research output: Contribution to journal › Article › peer-review

224 Scopus citations

Abstract

The in vitro bioassay for LH/hCG employing isolated rat testes has been significantly improved by utilization of collagenase-dispersed interstitial cells in the presence of phosphodiesterase inhibitors. The sensitivity of the assay system has been extended to 20µIU (2 picograms) of hCG, and the index of precision of the method (λ) is less than 0.04. The high sensitivity of the interstitial cell assay permits bioassay of circulating gonadotropins in human plasma.

Original language	English (US)
Pages (from-to)	610-613
Number of pages	4
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	39
Issue number	3
DOIs	https://doi.org/10.1210/jcem-39-3-610
State	Published - Sep 1974

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: Testosterone production by dispersed leydig cells

Abstract

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