A “hot Spot”-Enhanced paper lateral flow assay for ultrasensitive detection of traumatic brain injury biomarker S-100β in blood plasma

Xuefei Gao, Jennifer Boryczka, Peng Zheng, Sujan Kasani, Feng Yang, Elizabeth B. Engler-Chiurazzi, James W. Simpkins, Jane G. Wigginton, Nianqiang Wu

Research output: Contribution to journalArticlepeer-review

Abstract

Currently colorimetric paper lateral flow strips (PLFS) encounter two major limitations, that is, low sensitivity and severe interference from complex sample matrices such as blood. These shortcomings limit their application in detection of low-concentration analytes in complex samples. To solve these problems, a PLFS has been developed by utilizing surface-enhanced Raman scattering (SERS) for sensing signal transduction. In particular, a hierarchical three-dimensional nanostructure has been designed to create “hot spots”, which can significantly amplify the SERS sensing signal, leading to high sensitivity. As a result, this PLFS has demonstrated a limit of detection (LOD) of 5.0 pg mL−1 toward detection of S-100β, a traumatic brain injury (TBI) protein biomarker in blood plasma. The PLFS has been successfully used for rapid measurement of S-100β in clinical TBI patient samples taken in the emergency department. Availability of PLFS for blood testing would shift the paradigm of TBI patient management and clinical outcome in emergency departments. It is expected that this type of PLFS can be adapted for rapid detection of various human diseases due to its capability of measuring a low level of protein blood biomarkers in complex human fluids.

Original languageEnglish (US)
Article number112967
JournalBiosensors and Bioelectronics
Volume177
DOIs
StatePublished - Apr 1 2021

Keywords

  • Biomarker
  • Blood testing
  • Paper lateral flow assay
  • S-100β
  • Surface-enhanced Raman scattering
  • Traumatic brain injury

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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