A large T cell invagination with CD2 enrichment resets receptor engagement in the immunological synapse

Kentner Singleton, Nadia Parvaze, Kavyya R. Dama, Kenneth S. Chen, Paula Jennings, Bozidar Purtic, Michael D. Sjaastad, Christopher Gilpin, Mark M. Davis, Christoph Wülfing

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

T cell activation is driven by the TCR and complemented by costimulation. We have studied the dynamics of ligand-engagement of the costimulatory receptor CD2 in T cell/APC couples. Thousands of ligand-engaged CD2 molecules were included in a large T cell invagination at the center of the cellular interface within l min of cell couple formation. The structure and regulation of this invagination shared numerous features with phagocytosis and macropinocytosis. Tnree observations further characterize the invagination and the inclusion of CD2: 1) numerous ligand-engaged receptors were enriched in and internalized through the T cell invagination, none as prominently as CD2; 2) dissolution of the T cell invagination and CD2 engagement were required for effective proximal T cell signaling; and 3) the T cell invagination was uniquely sensitive to the affinity of the TCR for peptide-MHC. Based on this characterization, we speculate that the T cell invagination, aided by CD2 enrichment, internalizes parts of the TCR signaling machinery to reset T cell signaling upon agonist-mediated, stable APC contact.

Original languageEnglish (US)
Pages (from-to)4402-4413
Number of pages12
JournalJournal of Immunology
Volume177
Issue number7
DOIs
StatePublished - Oct 1 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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