A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno

Mark J. Snee, Dianne Harrison, Nan Yan, Paul M. Macdonald

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In Drosophila, posterior embryonic body patterning and germ cell formation rely on Oskar, a protein that is concentrated at the posterior pole of the oocyte. A program of mRNA localization and translational regulation ensures that Oskar is only expressed at the proper location. One key regulatory factor is Bruno, which represses translation of oskar mRNA before its localization. Ectopic expression of a bruno cDNA prolongs repression, even after oskar mRNA is localized, and posterior body patterning is efficiently and selectively blocked. Surprisingly, the initial accumulation of Oskar, while frequently reduced, is not eliminated, arguing that levels of Oskar previously thought to be sufficient for patterning do not suffice, or that Bruno acts at a downstream step in patterning. Expression of the bruno cDNA does not inhibit posterior patterning when Oskar is expressed independent of Bruno-mediated regulation, ruling out a downstream requirement for Bruno. Notably, an Oskar::GFP reporter protein reveals continual accumulation during the late phases of oogenesis. Taken together, these results strongly argue that a late phase in accumulation of Osk protein, typically not monitored because of imperviousness of late stage oocytes to antibodies, is crucial for body patterning.

Original languageEnglish (US)
Pages (from-to)246-255
Number of pages10
JournalDifferentiation
Volume75
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Body Patterning
Drosophila
Embryonic Structures
Oocytes
Complementary DNA
Oogenesis
Messenger RNA
Proteins
Protein Biosynthesis
Germ Cells
Antibodies
Ectopic Gene Expression

Keywords

  • Bruno
  • Drosophila
  • Gurken
  • Oogenesis
  • Oskar
  • Translational regulation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Developmental Biology
  • Cell Biology

Cite this

A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno. / Snee, Mark J.; Harrison, Dianne; Yan, Nan; Macdonald, Paul M.

In: Differentiation, Vol. 75, No. 3, 03.2007, p. 246-255.

Research output: Contribution to journalArticle

@article{388055f5a1d54155af69840232ebd10f,
title = "A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno",
abstract = "In Drosophila, posterior embryonic body patterning and germ cell formation rely on Oskar, a protein that is concentrated at the posterior pole of the oocyte. A program of mRNA localization and translational regulation ensures that Oskar is only expressed at the proper location. One key regulatory factor is Bruno, which represses translation of oskar mRNA before its localization. Ectopic expression of a bruno cDNA prolongs repression, even after oskar mRNA is localized, and posterior body patterning is efficiently and selectively blocked. Surprisingly, the initial accumulation of Oskar, while frequently reduced, is not eliminated, arguing that levels of Oskar previously thought to be sufficient for patterning do not suffice, or that Bruno acts at a downstream step in patterning. Expression of the bruno cDNA does not inhibit posterior patterning when Oskar is expressed independent of Bruno-mediated regulation, ruling out a downstream requirement for Bruno. Notably, an Oskar::GFP reporter protein reveals continual accumulation during the late phases of oogenesis. Taken together, these results strongly argue that a late phase in accumulation of Osk protein, typically not monitored because of imperviousness of late stage oocytes to antibodies, is crucial for body patterning.",
keywords = "Bruno, Drosophila, Gurken, Oogenesis, Oskar, Translational regulation",
author = "Snee, {Mark J.} and Dianne Harrison and Nan Yan and Macdonald, {Paul M.}",
year = "2007",
month = "3",
doi = "10.1111/j.1432-0436.2006.00136.x",
language = "English (US)",
volume = "75",
pages = "246--255",
journal = "Differentiation",
issn = "0301-4681",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno

AU - Snee, Mark J.

AU - Harrison, Dianne

AU - Yan, Nan

AU - Macdonald, Paul M.

PY - 2007/3

Y1 - 2007/3

N2 - In Drosophila, posterior embryonic body patterning and germ cell formation rely on Oskar, a protein that is concentrated at the posterior pole of the oocyte. A program of mRNA localization and translational regulation ensures that Oskar is only expressed at the proper location. One key regulatory factor is Bruno, which represses translation of oskar mRNA before its localization. Ectopic expression of a bruno cDNA prolongs repression, even after oskar mRNA is localized, and posterior body patterning is efficiently and selectively blocked. Surprisingly, the initial accumulation of Oskar, while frequently reduced, is not eliminated, arguing that levels of Oskar previously thought to be sufficient for patterning do not suffice, or that Bruno acts at a downstream step in patterning. Expression of the bruno cDNA does not inhibit posterior patterning when Oskar is expressed independent of Bruno-mediated regulation, ruling out a downstream requirement for Bruno. Notably, an Oskar::GFP reporter protein reveals continual accumulation during the late phases of oogenesis. Taken together, these results strongly argue that a late phase in accumulation of Osk protein, typically not monitored because of imperviousness of late stage oocytes to antibodies, is crucial for body patterning.

AB - In Drosophila, posterior embryonic body patterning and germ cell formation rely on Oskar, a protein that is concentrated at the posterior pole of the oocyte. A program of mRNA localization and translational regulation ensures that Oskar is only expressed at the proper location. One key regulatory factor is Bruno, which represses translation of oskar mRNA before its localization. Ectopic expression of a bruno cDNA prolongs repression, even after oskar mRNA is localized, and posterior body patterning is efficiently and selectively blocked. Surprisingly, the initial accumulation of Oskar, while frequently reduced, is not eliminated, arguing that levels of Oskar previously thought to be sufficient for patterning do not suffice, or that Bruno acts at a downstream step in patterning. Expression of the bruno cDNA does not inhibit posterior patterning when Oskar is expressed independent of Bruno-mediated regulation, ruling out a downstream requirement for Bruno. Notably, an Oskar::GFP reporter protein reveals continual accumulation during the late phases of oogenesis. Taken together, these results strongly argue that a late phase in accumulation of Osk protein, typically not monitored because of imperviousness of late stage oocytes to antibodies, is crucial for body patterning.

KW - Bruno

KW - Drosophila

KW - Gurken

KW - Oogenesis

KW - Oskar

KW - Translational regulation

UR - http://www.scopus.com/inward/record.url?scp=33947204064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947204064&partnerID=8YFLogxK

U2 - 10.1111/j.1432-0436.2006.00136.x

DO - 10.1111/j.1432-0436.2006.00136.x

M3 - Article

C2 - 17359300

AN - SCOPUS:33947204064

VL - 75

SP - 246

EP - 255

JO - Differentiation

JF - Differentiation

SN - 0301-4681

IS - 3

ER -