Abstract
Our previous studies have shown that DNase I hypersensitive sites 1 and 2 (HS1-2) and HS3-6 within the mouse Vκ-Jκ intervening region are essential for controlling locus contraction and creating a diverse Ab repertoire. In this article, we demonstrate that a 6.3-κb deletion encompassing HS1-6 altogether not only leads to the predictable sums of these phenotypes, but also results in a novel hyperelevation of transcription of proximal Vk genes, in both pre-B and splenic B cells. These findings reveal previously unrecognized additional functions for cis-elements within the Vκ-Jκ intervening region, namely, prevention of the production of massive levels of noncoding RNA species by silencing transcription of germline proximal Vk genes in both developing and mature B cells.
Original language | English (US) |
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Pages (from-to) | 3746-3754 |
Number of pages | 9 |
Journal | Journal of Immunology |
Volume | 193 |
Issue number | 7 |
DOIs | |
State | Published - Oct 1 2014 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology