A major deletion in the Vκ-Jκ intervening region results in hyperelevated transcription of proximal Vκ genes and a severely restricted repertoire

Yougui Xiang, Sung Kyun Park, William T. Garrard

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Our previous studies have shown that DNase I hypersensitive sites 1 and 2 (HS1-2) and HS3-6 within the mouse Vκ-Jκ intervening region are essential for controlling locus contraction and creating a diverse Ab repertoire. In this article, we demonstrate that a 6.3-κb deletion encompassing HS1-6 altogether not only leads to the predictable sums of these phenotypes, but also results in a novel hyperelevation of transcription of proximal Vk genes, in both pre-B and splenic B cells. These findings reveal previously unrecognized additional functions for cis-elements within the Vκ-Jκ intervening region, namely, prevention of the production of massive levels of noncoding RNA species by silencing transcription of germline proximal Vk genes in both developing and mature B cells.

Original languageEnglish (US)
Pages (from-to)3746-3754
Number of pages9
JournalJournal of Immunology
Volume193
Issue number7
DOIs
StatePublished - Oct 1 2014

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B-Lymphocytes
Untranslated RNA
Deoxyribonuclease I
RNA Interference
Genes
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

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A major deletion in the Vκ-Jκ intervening region results in hyperelevated transcription of proximal Vκ genes and a severely restricted repertoire. / Xiang, Yougui; Park, Sung Kyun; Garrard, William T.

In: Journal of Immunology, Vol. 193, No. 7, 01.10.2014, p. 3746-3754.

Research output: Contribution to journalArticle

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