A mechanism of cell death involving an adenylyl cyclase/PKA signaling pathway is induced by the Cry1Ab toxin of Bacillus thuringiensis

Xuebin Zhang, Mehmet Candas, Natalya B. Griko, Ronald Taussig, Lee A. Bulla

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Many pathogenic organisms and their toxins target host cell receptors, the consequence of which is altered signaling events that lead to aberrant activity or cell death. A significant body of literature describes various molecular and cellular aspects of toxins associated with bacterial invasion, colonization, and host cell disruption. However, there is little information on the molecular and cellular mechanisms associated with the insecticidal action of Bacillus thuringiensis (Bt) Cry toxins. Recently, we reported that the Cry1Ab toxin produced by Bt kills insect cells by activating a Mg2+-dependent cytotoxic event upon binding of the toxin to its receptor BT-R1. Here we show that binding of Cry toxin to BT-R1 provokes cell death by activating a previously undescribed signaling pathway involving stimulation of G protein (Gαs) and adenylyl cyclase, increased cAMP levels, and activation of protein kinase A. Induction of the adenylyl cyclase/protein kinase A pathway is manifested by sequential cytological changes that include membrane blebbing, appearance of ghost nuclei, cell swelling, and lysis. The discovery of a toxin-induced cell death pathway specifically linked to BT-R 1 in insect cells should provide insights into how insects evolve resistance to Bt and into the development of new, safer insecticides.

Original languageEnglish (US)
Pages (from-to)9897-9902
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number26
DOIs
StatePublished - Jun 27 2006

Fingerprint

Bacillus thuringiensis
Adenylyl Cyclases
Cell Death
Insects
Cyclic AMP-Dependent Protein Kinases
Blister
Insecticides
Cell Nucleus
GTP-Binding Proteins
Membranes

Keywords

  • Cry toxin
  • Protein kinase a cadherin receptor cAMP
  • Signal transduction

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A mechanism of cell death involving an adenylyl cyclase/PKA signaling pathway is induced by the Cry1Ab toxin of Bacillus thuringiensis. / Zhang, Xuebin; Candas, Mehmet; Griko, Natalya B.; Taussig, Ronald; Bulla, Lee A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 26, 27.06.2006, p. 9897-9902.

Research output: Contribution to journalArticle

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