A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry

J. D. Nunes-Miranda, Cristina Núñez, Hugo M. Santos, G. Vale, Miguel Reboiro-Jato, Florentino Fdez-Riverola, Carlos Lodeiro, Manuel Miró, J. L. Capelo

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The integration of ultrasound (US)-assisted sample processing on-chip in a lab-on-a-valve (LOV) format for automated high-throughput shotgun proteomic assays is herein presented for the first time. The proof of concept of this system was demonstrated with the analysis of three proteins and sera from patients with lymphoma or myeloma.

Original languageEnglish (US)
Pages (from-to)992-995
Number of pages4
JournalAnalyst
Volume139
Issue number5
DOIs
StatePublished - Feb 3 2014

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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