Metod opredeleniia aktivnosti o-difenoloksidazy v reaktsii okisleniia pirokatekhina.

Translated title of the contribution: A method for determining theo-diphenoloxidase activity in the pyrocatechol oxidation reaction

I. A. Butovich, V. A. Tertykh

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A new simple and sensitive spectrophotometric method is suggested for determining the catecholase activity of diphenoloxidase. The method is based on the enzymatic oxidation of pyrocatechol to 1,2-benzoquinone (BQ) in the presence of the excess of ethylenediamine sulphate (EDA). The condensation product (products) of BQ and EDA (P365) is stable in the solution and possesses strong absorption in the range of 365 nm. The molar absorption factor, E365 (under condition that the molar reaction ratio of catechol to P365 is 1:1) is 15500 M-1 cm-1 on the average. Optimal reaction conditions (pH 7.0, T=25-30 degrees C, [EDA]o = 5 mg/ml) are determined. The advantages and restrictions of the suggested technique in comparison with the methods described earlier are discussed.

Translated title of the contributionA method for determining theo-diphenoloxidase activity in the pyrocatechol oxidation reaction
Original languageRussian
Pages (from-to)437-441
Number of pages5
JournalUkrainskii biokhimicheskii zhurnal
Volume56
Issue number4
StatePublished - Jul 1 1984

ASJC Scopus subject areas

  • Biochemistry

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