A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium

J. K V Willson, D. E. Luberoff, A. Pitts, T. G. Pretlow

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Abstract

Several methods for the dissociation of human tonsils into cell suspensions were compared. Dissociation of tonsils using 0.25% trypsin gave both the largest number of total cells, and the largest number of plasma cells per gram of tonsil. Lymphocytes and plasma cells were separated in a previously described isokinetic gradient of Ficoll in tissue culture medium. In the purest gradient fractions, lymphocytes were 97.2% ± 1.9% of nucleated cells. The purest gradient fractions contained 43.1% ± 5.9% plasma cells. More than 95% of purified lymphocytes and plasma cells excluded Trypan Blue.

Original languageEnglish (US)
Pages (from-to)161-170
Number of pages10
JournalImmunology
Volume28
Issue number1
StatePublished - 1975

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Ficoll
Palatine Tonsil
Plasma Cells
Culture Media
Lymphocytes
Trypan Blue
Trypsin
Suspensions
Cell Count

ASJC Scopus subject areas

  • Immunology

Cite this

A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium. / Willson, J. K V; Luberoff, D. E.; Pitts, A.; Pretlow, T. G.

In: Immunology, Vol. 28, No. 1, 1975, p. 161-170.

Research output: Contribution to journalArticle

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