A method to study the impact of chemically-induced ovarian failure on exercise capacity and cardiac adaptation in mice

Hao Chen, Jessica N. Perez, Eleni Constantopoulos, Laurel McKee, Jessica Regan, Patricia B. Hoyer, Heddwen L. Brooks, John Konhilas

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The risk of cardiovascular disease (CVD) increases in post-menopausal women, yet, the role of exercise, as a preventative measure for CVD risk in post-menopausal women has not been adequately studied. Accordingly, we investigated the impact of voluntary cage-wheel exercise and forced treadmill exercise on cardiac adaptation in menopausal mice. The most commonly used inducible model for mimicking menopause in women is the ovariectomized (OVX) rodent. However, the OVX model has a few dissimilarities from menopause in humans. In this study, we administered 4-vinylcyclohexene diepoxide (VCD) to female mice, which accelerates ovarian failure as an alternative menopause model to study the impact of exercise in menopausal mice. VCD selectively accelerates the loss of primary and primordial follicles resulting in an endocrine state that closely mimics the natural progression from pre- to peri- to post-menopause in humans. To determine the impact of exercise on exercise capacity and cardiac adaptation in VCD-treated female mice, two methods were used. First, we exposed a group of VCD-treated and untreated mice to a voluntary cage wheel. Second, we used forced treadmill exercise to determine exercise capacity in a separate group VCD-treated and untreated mice measured as a tolerance to exercise intensity and endurance.

Original languageEnglish (US)
Article numbere51083
JournalJournal of Visualized Experiments
Issue number86
DOIs
StatePublished - Apr 7 2014
Externally publishedYes

Fingerprint

Exercise equipment
Exercise
Wheels
Menopause
Durability
Cardiovascular Diseases
Postmenopause
Exercise Tolerance
4-vinyl-1-cyclohexene dioxide
Rodentia

Keywords

  • Adaptive cardiac adaptation
  • Exercise capacity
  • Forced treadmill exercise
  • Issue 86
  • Medicine
  • Menopause
  • VCD
  • Voluntary wheel running

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A method to study the impact of chemically-induced ovarian failure on exercise capacity and cardiac adaptation in mice. / Chen, Hao; Perez, Jessica N.; Constantopoulos, Eleni; McKee, Laurel; Regan, Jessica; Hoyer, Patricia B.; Brooks, Heddwen L.; Konhilas, John.

In: Journal of Visualized Experiments, No. 86, e51083, 07.04.2014.

Research output: Contribution to journalArticle

Chen, Hao ; Perez, Jessica N. ; Constantopoulos, Eleni ; McKee, Laurel ; Regan, Jessica ; Hoyer, Patricia B. ; Brooks, Heddwen L. ; Konhilas, John. / A method to study the impact of chemically-induced ovarian failure on exercise capacity and cardiac adaptation in mice. In: Journal of Visualized Experiments. 2014 ; No. 86.
@article{9af9b7e03a3745d7b05593e09c183b20,
title = "A method to study the impact of chemically-induced ovarian failure on exercise capacity and cardiac adaptation in mice",
abstract = "The risk of cardiovascular disease (CVD) increases in post-menopausal women, yet, the role of exercise, as a preventative measure for CVD risk in post-menopausal women has not been adequately studied. Accordingly, we investigated the impact of voluntary cage-wheel exercise and forced treadmill exercise on cardiac adaptation in menopausal mice. The most commonly used inducible model for mimicking menopause in women is the ovariectomized (OVX) rodent. However, the OVX model has a few dissimilarities from menopause in humans. In this study, we administered 4-vinylcyclohexene diepoxide (VCD) to female mice, which accelerates ovarian failure as an alternative menopause model to study the impact of exercise in menopausal mice. VCD selectively accelerates the loss of primary and primordial follicles resulting in an endocrine state that closely mimics the natural progression from pre- to peri- to post-menopause in humans. To determine the impact of exercise on exercise capacity and cardiac adaptation in VCD-treated female mice, two methods were used. First, we exposed a group of VCD-treated and untreated mice to a voluntary cage wheel. Second, we used forced treadmill exercise to determine exercise capacity in a separate group VCD-treated and untreated mice measured as a tolerance to exercise intensity and endurance.",
keywords = "Adaptive cardiac adaptation, Exercise capacity, Forced treadmill exercise, Issue 86, Medicine, Menopause, VCD, Voluntary wheel running",
author = "Hao Chen and Perez, {Jessica N.} and Eleni Constantopoulos and Laurel McKee and Jessica Regan and Hoyer, {Patricia B.} and Brooks, {Heddwen L.} and John Konhilas",
year = "2014",
month = "4",
day = "7",
doi = "10.3791/51083",
language = "English (US)",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "86",

}

TY - JOUR

T1 - A method to study the impact of chemically-induced ovarian failure on exercise capacity and cardiac adaptation in mice

AU - Chen, Hao

AU - Perez, Jessica N.

AU - Constantopoulos, Eleni

AU - McKee, Laurel

AU - Regan, Jessica

AU - Hoyer, Patricia B.

AU - Brooks, Heddwen L.

AU - Konhilas, John

PY - 2014/4/7

Y1 - 2014/4/7

N2 - The risk of cardiovascular disease (CVD) increases in post-menopausal women, yet, the role of exercise, as a preventative measure for CVD risk in post-menopausal women has not been adequately studied. Accordingly, we investigated the impact of voluntary cage-wheel exercise and forced treadmill exercise on cardiac adaptation in menopausal mice. The most commonly used inducible model for mimicking menopause in women is the ovariectomized (OVX) rodent. However, the OVX model has a few dissimilarities from menopause in humans. In this study, we administered 4-vinylcyclohexene diepoxide (VCD) to female mice, which accelerates ovarian failure as an alternative menopause model to study the impact of exercise in menopausal mice. VCD selectively accelerates the loss of primary and primordial follicles resulting in an endocrine state that closely mimics the natural progression from pre- to peri- to post-menopause in humans. To determine the impact of exercise on exercise capacity and cardiac adaptation in VCD-treated female mice, two methods were used. First, we exposed a group of VCD-treated and untreated mice to a voluntary cage wheel. Second, we used forced treadmill exercise to determine exercise capacity in a separate group VCD-treated and untreated mice measured as a tolerance to exercise intensity and endurance.

AB - The risk of cardiovascular disease (CVD) increases in post-menopausal women, yet, the role of exercise, as a preventative measure for CVD risk in post-menopausal women has not been adequately studied. Accordingly, we investigated the impact of voluntary cage-wheel exercise and forced treadmill exercise on cardiac adaptation in menopausal mice. The most commonly used inducible model for mimicking menopause in women is the ovariectomized (OVX) rodent. However, the OVX model has a few dissimilarities from menopause in humans. In this study, we administered 4-vinylcyclohexene diepoxide (VCD) to female mice, which accelerates ovarian failure as an alternative menopause model to study the impact of exercise in menopausal mice. VCD selectively accelerates the loss of primary and primordial follicles resulting in an endocrine state that closely mimics the natural progression from pre- to peri- to post-menopause in humans. To determine the impact of exercise on exercise capacity and cardiac adaptation in VCD-treated female mice, two methods were used. First, we exposed a group of VCD-treated and untreated mice to a voluntary cage wheel. Second, we used forced treadmill exercise to determine exercise capacity in a separate group VCD-treated and untreated mice measured as a tolerance to exercise intensity and endurance.

KW - Adaptive cardiac adaptation

KW - Exercise capacity

KW - Forced treadmill exercise

KW - Issue 86

KW - Medicine

KW - Menopause

KW - VCD

KW - Voluntary wheel running

UR - http://www.scopus.com/inward/record.url?scp=84940233112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940233112&partnerID=8YFLogxK

U2 - 10.3791/51083

DO - 10.3791/51083

M3 - Article

C2 - 24747886

AN - SCOPUS:84940233112

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 86

M1 - e51083

ER -