A microfluidic assay for metastasis potential analysis

Smitha M N Rao, Uday Tata, Victor K. Lin, Jer Tsong Hsieh, Kytai Nguyen, J. C. Chiao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have designed and characterized a poly-dimethylsiloxane (PDMS) based microfluidic device called MiMiC™ that enables time-lapse study of cell migration. Cell migration is a key step of malignant metastasis during cancer progression. The device mimics the narrow confines the cells need to traverse and the microenvironments that are similar to the ones inside human body. Photolithography and soft lithography processes were used to fabricate the microfluidic devices. The device consists of two separate chambers connected by microfluidic channels allowing introduction of cells in one chamber and chemoattractants in the other. The response of lung-metastasized prostate cancer (PC -3-ML) cells and their migration response to chemoattractants were observed and analyzed. The numbers of cells under migration were determined from time-lapse images and compared to control groups. Our microfluidic assays provide advantages over the traditional Boyden chambers such as time-lapse observation, use of smaller amounts of reagents and direct assessment of cells under migration.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
Pages143-144
Number of pages2
StatePublished - 2010
Event1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, United States
Duration: Feb 7 2010Feb 10 2010

Other

Other1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
CountryUnited States
CityHouston, TX
Period2/7/102/10/10

Fingerprint

Microfluidics
Cell Movement
Lab-On-A-Chip Devices
Assays
Neoplasm Metastasis
Chemotactic Factors
Photolithography
Polydimethylsiloxane
Equipment and Supplies
Lithography
Human Body
Prostatic Neoplasms
Observation
Lung
Control Groups
Neoplasms

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)

Cite this

Rao, S. M. N., Tata, U., Lin, V. K., Hsieh, J. T., Nguyen, K., & Chiao, J. C. (2010). A microfluidic assay for metastasis potential analysis. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010 (pp. 143-144)

A microfluidic assay for metastasis potential analysis. / Rao, Smitha M N; Tata, Uday; Lin, Victor K.; Hsieh, Jer Tsong; Nguyen, Kytai; Chiao, J. C.

Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. 2010. p. 143-144.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rao, SMN, Tata, U, Lin, VK, Hsieh, JT, Nguyen, K & Chiao, JC 2010, A microfluidic assay for metastasis potential analysis. in Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. pp. 143-144, 1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010, Houston, TX, United States, 2/7/10.
Rao SMN, Tata U, Lin VK, Hsieh JT, Nguyen K, Chiao JC. A microfluidic assay for metastasis potential analysis. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. 2010. p. 143-144
Rao, Smitha M N ; Tata, Uday ; Lin, Victor K. ; Hsieh, Jer Tsong ; Nguyen, Kytai ; Chiao, J. C. / A microfluidic assay for metastasis potential analysis. Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. 2010. pp. 143-144
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