A microfluidic assay for metastasis potential analysis

Smitha M N Rao, Uday Tata, Victor K. Lin, Jer Tsong Hsieh, Kytai Nguyen, J. C. Chiao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have designed and characterized a poly-dimethylsiloxane (PDMS) based microfluidic device called MiMiC™ that enables time-lapse study of cell migration. Cell migration is a key step of malignant metastasis during cancer progression. The device mimics the narrow confines the cells need to traverse and the microenvironments that are similar to the ones inside human body. Photolithography and soft lithography processes were used to fabricate the microfluidic devices. The device consists of two separate chambers connected by microfluidic channels allowing introduction of cells in one chamber and chemoattractants in the other. The response of lung-metastasized prostate cancer (PC -3-ML) cells and their migration response to chemoattractants were observed and analyzed. The numbers of cells under migration were determined from time-lapse images and compared to control groups. Our microfluidic assays provide advantages over the traditional Boyden chambers such as time-lapse observation, use of smaller amounts of reagents and direct assessment of cells under migration.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
PublisherASME
Pages143-144
Number of pages2
ISBN (Print)9780791843925
DOIs
StatePublished - Jan 1 2010
Event1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, United States
Duration: Feb 7 2010Feb 10 2010

Publication series

NameProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010

Other

Other1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
CountryUnited States
CityHouston, TX
Period2/7/102/10/10

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)

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  • Cite this

    Rao, S. M. N., Tata, U., Lin, V. K., Hsieh, J. T., Nguyen, K., & Chiao, J. C. (2010). A microfluidic assay for metastasis potential analysis. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010 (pp. 143-144). (Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010). ASME. https://doi.org/10.1115/nemb2010-13300