A microtubule depolymerizing kinesin functions during both flagellar disassembly and flagellar assembly in Chlamydomonas

Tian Piao, Minna Luo, Liang Wang, Yan Guo, De Li, Peng Li, William J. Snell, Junmin Pan

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Cilia and flagella are dynamic organelles that are assembled and disassembled during cell differentiation, during stress, and during the cell cycle. Although intraflagellar transport (IFT) is well documented to be responsible for transport of ciliary/flagellar precursors from the cell body to the flagella, little is known about the molecular mechanisms for mobilizing the cell body-localized precursors to make them available for transport during organelle assembly or for disassembling the microtubule-based axoneme during shortening. Here, we show that Chlamydomonas kinesin-13 (CrKinesin-13), a member of the kinesin-13 family of microtubule depolymerizing kinesins best known for their roles in the cell cycle, functions in flagellar disassembly and flagellar assembly. Activation of a cell to generate new flagella induces rapid phosphorylation of CrKinesin-13, and activation of flagellar shortening induces the immediate transport of CrKinesin-13 via intraflagellar transport from the cell body into the flagella. Cells depleted of CrKinesin-13 by RNAi assemble flagella after cell division but are incapable of the rapid assembly of flagella that normally occurs after flagellar detachment. Furthermore, they are inhibited in flagellar shortening. Thus, CrKinesin-13 is dynamically regulated during flagellar assembly and disassembly in Chlamydomonas and functions in each.

Original languageEnglish (US)
Pages (from-to)4713-4718
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number12
DOIs
StatePublished - Mar 24 2009

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Chlamydomonas
Kinesin
Flagella
Microtubules
Organelles
Cell Cycle
Axoneme
Cilia
RNA Interference
Cell Division
Cell Differentiation
Phosphorylation
Cell Body

Keywords

  • Cilia
  • Flagella
  • Intraflagellar transport
  • Kinesin-13

ASJC Scopus subject areas

  • General

Cite this

A microtubule depolymerizing kinesin functions during both flagellar disassembly and flagellar assembly in Chlamydomonas. / Piao, Tian; Luo, Minna; Wang, Liang; Guo, Yan; Li, De; Li, Peng; Snell, William J.; Pan, Junmin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 12, 24.03.2009, p. 4713-4718.

Research output: Contribution to journalArticle

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AU - Li, Peng

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