A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1)

G. C. Mishra, M. T. Berton, K. G. Oliver, P. H. Krammer, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

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Abstract

This study describes the generation of a monoclonal mouse x rat antibody (G-48) that recognizes a determinant on the serologically defined LFA-1 α-chain. It immunoprecipitates two noncovalently associated polypeptides of 176,000 and 95,000 M(r) respectively from lysates of radioiodinated BCL1 cells, T cells, and B cells. G-48 mimics the biological actions of BSF-1 by inducing increased levels of Ia antigen expression on resting B cells, augmenting the proliferation of anti-δ-stimulated B cells, and in insolubilized form, inducing IgG1 secretion by LPS-activated B cells. G-48 does not have BCDFμ, BCGF II, nor IL 2 activity. These results demonstrate that LFA-1 plays an important role in B cell activation, proliferation, and differentiation.

Original languageEnglish (US)
Pages (from-to)1590-1598
Number of pages9
JournalJournal of Immunology
Volume137
Issue number5
StatePublished - 1986

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Interleukin-4
B-Lymphocytes
Lymphocyte Function-Associated Antigen-1
Cell Proliferation
Histocompatibility Antigens Class II
Interleukin-5
Interleukin-2
Cell Differentiation
Immunoglobulin G
anti-LFA-1 monoclonal antibody
T-Lymphocytes
Peptides
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Mishra, G. C., Berton, M. T., Oliver, K. G., Krammer, P. H., Uhr, J. W., & Vitetta, E. S. (1986). A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1). Journal of Immunology, 137(5), 1590-1598.

A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1). / Mishra, G. C.; Berton, M. T.; Oliver, K. G.; Krammer, P. H.; Uhr, J. W.; Vitetta, E. S.

In: Journal of Immunology, Vol. 137, No. 5, 1986, p. 1590-1598.

Research output: Contribution to journalArticle

Mishra, GC, Berton, MT, Oliver, KG, Krammer, PH, Uhr, JW & Vitetta, ES 1986, 'A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1)', Journal of Immunology, vol. 137, no. 5, pp. 1590-1598.
Mishra, G. C. ; Berton, M. T. ; Oliver, K. G. ; Krammer, P. H. ; Uhr, J. W. ; Vitetta, E. S. / A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1). In: Journal of Immunology. 1986 ; Vol. 137, No. 5. pp. 1590-1598.
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