A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1)

G. C. Mishra, M. T. Berton, K. G. Oliver, P. H. Krammer, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

This study describes the generation of a monoclonal mouse x rat antibody (G-48) that recognizes a determinant on the serologically defined LFA-1 α-chain. It immunoprecipitates two noncovalently associated polypeptides of 176,000 and 95,000 M(r) respectively from lysates of radioiodinated BCL1 cells, T cells, and B cells. G-48 mimics the biological actions of BSF-1 by inducing increased levels of Ia antigen expression on resting B cells, augmenting the proliferation of anti-δ-stimulated B cells, and in insolubilized form, inducing IgG1 secretion by LPS-activated B cells. G-48 does not have BCDFμ, BCGF II, nor IL 2 activity. These results demonstrate that LFA-1 plays an important role in B cell activation, proliferation, and differentiation.

Original languageEnglish (US)
Pages (from-to)1590-1598
Number of pages9
JournalJournal of Immunology
Volume137
Issue number5
StatePublished - Nov 19 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1)'. Together they form a unique fingerprint.

  • Cite this

    Mishra, G. C., Berton, M. T., Oliver, K. G., Krammer, P. H., Uhr, J. W., & Vitetta, E. S. (1986). A monoclonal anti-mouse LFA-1α antibody mimics the biological effects of B cell stimulatory factor-1 (BSF-1). Journal of Immunology, 137(5), 1590-1598.