A Munc13/RIM/Rab3 tripartite complex: From priming to plasticity?

Irina Dulubova, Xuelin Lou, Jun Lu, Iryna Huryeva, Amer Alam, Ralf Schneggenburger, Thomas C. Südhof, Jose Rizo-Rey

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

α-RIMs and Munc13s are active zone proteins that control priming of synaptic vesicles to a readily releasable state, and interact with each other via their N-terminal sequences. The α-RIM N-terminal sequence also binds to Rab3s (small synaptic vesicle GTPases), an interaction that regulates presynaptic plasticity. We now demonstrate that α-RIMs contain adjacent but separate Munc13- and Rab3-binding sites, allowing formation of a tripartite Rab3/RIM/Munc13 complex. Munc13 binding is mediated by the α-RIM zinc-finger domain. Elucidation of the three-dimensional structure of this domain by NMR spectroscopy facilitated the design of a mutation that abolishes α-RIM/Munc13 binding. Selective disruption of this interaction in the calyx of Held synapse decreased the size of the readily releasable vesicle pool. Our data suggest that the ternary Rab3/RIM/Munc13 interaction approximates synaptic vesicles to the priming machinery, providing a substrate for presynaptic plasticity. The modular architecture of α-RIMs, with nested binding sites for Rab3 and other targets, may be a general feature of Rab effectors that share homology with the α-RIM N-terminal sequence.

Original languageEnglish (US)
Pages (from-to)2839-2850
Number of pages12
JournalEMBO Journal
Volume24
Issue number16
DOIs
StatePublished - Aug 17 2005

Fingerprint

Reaction injection molding
Synaptic Vesicles
Plasticity
Binding Sites
GTP Phosphohydrolases
Zinc Fingers
Synapses
Magnetic Resonance Spectroscopy
Mutation
Proteins
Nuclear magnetic resonance spectroscopy
Machinery
Zinc

Keywords

  • Munc13/unc13
  • Neurotransmitter release
  • Protein NMR
  • Rab proteins
  • RIM

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Dulubova, I., Lou, X., Lu, J., Huryeva, I., Alam, A., Schneggenburger, R., ... Rizo-Rey, J. (2005). A Munc13/RIM/Rab3 tripartite complex: From priming to plasticity? EMBO Journal, 24(16), 2839-2850. https://doi.org/10.1038/sj.emboj.7600753

A Munc13/RIM/Rab3 tripartite complex : From priming to plasticity? / Dulubova, Irina; Lou, Xuelin; Lu, Jun; Huryeva, Iryna; Alam, Amer; Schneggenburger, Ralf; Südhof, Thomas C.; Rizo-Rey, Jose.

In: EMBO Journal, Vol. 24, No. 16, 17.08.2005, p. 2839-2850.

Research output: Contribution to journalArticle

Dulubova, I, Lou, X, Lu, J, Huryeva, I, Alam, A, Schneggenburger, R, Südhof, TC & Rizo-Rey, J 2005, 'A Munc13/RIM/Rab3 tripartite complex: From priming to plasticity?', EMBO Journal, vol. 24, no. 16, pp. 2839-2850. https://doi.org/10.1038/sj.emboj.7600753
Dulubova I, Lou X, Lu J, Huryeva I, Alam A, Schneggenburger R et al. A Munc13/RIM/Rab3 tripartite complex: From priming to plasticity? EMBO Journal. 2005 Aug 17;24(16):2839-2850. https://doi.org/10.1038/sj.emboj.7600753
Dulubova, Irina ; Lou, Xuelin ; Lu, Jun ; Huryeva, Iryna ; Alam, Amer ; Schneggenburger, Ralf ; Südhof, Thomas C. ; Rizo-Rey, Jose. / A Munc13/RIM/Rab3 tripartite complex : From priming to plasticity?. In: EMBO Journal. 2005 ; Vol. 24, No. 16. pp. 2839-2850.
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