A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation

Feng Zhang, Zhiyan Liang, Naoto Matsuki, Luc Van Kaer, Sebastian Joyce, Edward K. Wakeland, Thomas M. Aune

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.

Original languageEnglish (US)
Pages (from-to)4613-4620
Number of pages8
JournalJournal of Immunology
Volume171
Issue number9
StatePublished - Oct 1 2003

Fingerprint

Chromosomes, Human, Pair 18
Natural Killer T-Cells
Cell Differentiation
Homeostasis
Quantitative Trait Loci
Th1-Th2 Balance
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 5
T-Lymphocyte Subsets
Adaptive Immunity
Spleen
Cell Culture Techniques
T-Lymphocytes
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Zhang, F., Liang, Z., Matsuki, N., Van Kaer, L., Joyce, S., Wakeland, E. K., & Aune, T. M. (2003). A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation. Journal of Immunology, 171(9), 4613-4620.

A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation. / Zhang, Feng; Liang, Zhiyan; Matsuki, Naoto; Van Kaer, Luc; Joyce, Sebastian; Wakeland, Edward K.; Aune, Thomas M.

In: Journal of Immunology, Vol. 171, No. 9, 01.10.2003, p. 4613-4620.

Research output: Contribution to journalArticle

Zhang, F, Liang, Z, Matsuki, N, Van Kaer, L, Joyce, S, Wakeland, EK & Aune, TM 2003, 'A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation', Journal of Immunology, vol. 171, no. 9, pp. 4613-4620.
Zhang F, Liang Z, Matsuki N, Van Kaer L, Joyce S, Wakeland EK et al. A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation. Journal of Immunology. 2003 Oct 1;171(9):4613-4620.
Zhang, Feng ; Liang, Zhiyan ; Matsuki, Naoto ; Van Kaer, Luc ; Joyce, Sebastian ; Wakeland, Edward K. ; Aune, Thomas M. / A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation. In: Journal of Immunology. 2003 ; Vol. 171, No. 9. pp. 4613-4620.
@article{366a1860e64e45c4ac81b96bd65bdd26,
title = "A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation",
abstract = "Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to {"}repolarize{"} spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.",
author = "Feng Zhang and Zhiyan Liang and Naoto Matsuki and {Van Kaer}, Luc and Sebastian Joyce and Wakeland, {Edward K.} and Aune, {Thomas M.}",
year = "2003",
month = "10",
day = "1",
language = "English (US)",
volume = "171",
pages = "4613--4620",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

TY - JOUR

T1 - A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation

AU - Zhang, Feng

AU - Liang, Zhiyan

AU - Matsuki, Naoto

AU - Van Kaer, Luc

AU - Joyce, Sebastian

AU - Wakeland, Edward K.

AU - Aune, Thomas M.

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.

AB - Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.

UR - http://www.scopus.com/inward/record.url?scp=0142179993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142179993&partnerID=8YFLogxK

M3 - Article

C2 - 14568935

AN - SCOPUS:0142179993

VL - 171

SP - 4613

EP - 4620

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -