A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation

Feng Zhang, Zhiyan Liang, Naoto Matsuki, Luc Van Kaer, Sebastian Joyce, Edward K. Wakeland, Thomas M. Aune

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.

Original languageEnglish (US)
Pages (from-to)4613-4620
Number of pages8
JournalJournal of Immunology
Volume171
Issue number9
DOIs
StatePublished - Oct 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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