Abstract
Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4613-4620 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 171 |
| Issue number | 9 |
| State | Published - Oct 1 2003 |
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ASJC Scopus subject areas
- Immunology
Cite this
A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation. / Zhang, Feng; Liang, Zhiyan; Matsuki, Naoto; Van Kaer, Luc; Joyce, Sebastian; Wakeland, Edward K.; Aune, Thomas M.
In: Journal of Immunology, Vol. 171, No. 9, 01.10.2003, p. 4613-4620.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Murine Locus on Chromosome 18 Controls NKT Cell Homeostasis and Th Cell Differentiation
AU - Zhang, Feng
AU - Liang, Zhiyan
AU - Matsuki, Naoto
AU - Van Kaer, Luc
AU - Joyce, Sebastian
AU - Wakeland, Edward K.
AU - Aune, Thomas M.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.
AB - Th cell differentiation is a critical event in the adaptive immune response. C57BL strains develop predominant Th1 responses while BALB/c develops a predominant Th2 response. To identify quantitative trait loci controlling this variation, we performed Th1/Th2 differentiation assays of F1 x BALB/c progeny. A single strong quantitative trait locus was identified on chromosome 18, with weaker effects detectable on chromosomes 5, 12, and 14. By preparing a congenic BALB.B10.D2c18 strain, we were able to demonstrate that this single locus was sufficient to "repolarize" spleen cell cultures. This difference was not due to intrinsic differences in CD4+ T cells. Rather, introgression of the chromosome 18 locus into BALB/c disrupted Va14Ja18 NKT cell homeostasis resulting in the almost complete absence of this T cell subset. Taken together, these data indicate that genes within chromosome 18 control strain-dependent development of Va14ja18 NKT cells.
UR - http://www.scopus.com/inward/record.url?scp=0142179993&partnerID=8YFLogxK
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M3 - Article
C2 - 14568935
AN - SCOPUS:0142179993
VL - 171
SP - 4613
EP - 4620
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -