A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells

Nicholas G. James, Michelle A. Digman, Justin A. Ross, Barbara Barylko, Lei Wang, Jinhui Li, Yan Chen, Joachim D. Mueller, Enrico Gratton, Joseph P. Albanesi, David M. Jameson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background Dynamin 2 (Dyn2) is a ~ 100 kDa GTPase that assembles around the necks of nascent endocytic and Golgi vesicles and catalyzes membrane scission. Mutations in Dyn2 that cause centronuclear myopathy (CNM) have been shown to stabilize Dyn2 polymers against GTP-dependent disassembly in vitro. Precisely timed regulation of assembly and disassembly is believed to be critical for Dyn2 function in membrane vesiculation, and the CNM mutations interfere with this regulation by shifting the equilibrium toward the assembled state. Methods In this study we use two fluorescence fluctuation spectroscopy (FFS) approaches to show that a CNM mutant form of Dyn2 also has a greater propensity to self-assemble in the cytosol and on the plasma membrane of living cells. Results Results obtained using brightness analysis indicate that unassembled wild-type Dyn2 is predominantly tetrameric in the cytosol, although different oligomeric species are observed, depending on the concentration of expressed protein. In contrast, an R369W mutant identified in CNM patients forms higher-order oligomers at concentrations above 1 μM. Investigation of Dyn2-R369W by Total Internal Reflection Fluorescence (TIRF) FFS reveals that this mutant forms larger and more stable clathrin-containing structures on the plasma membrane than wild-type Dyn2. Conclusions and general significance These observations may explain defects in membrane trafficking reported in CNM patient cells and in heterologous systems expressing CNM-associated Dyn2 mutants.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1840
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Dynamin II
Congenital Structural Myopathies
Mutation
Fluorescence
Fluorescence Spectrometry
Cell membranes
Membranes
Cytosol
Cell Membrane
Spectroscopy
Transport Vesicles
Clathrin
GTP Phosphohydrolases
Guanosine Triphosphate
Oligomers
Luminance
Polymers
Cells

Keywords

  • Centronuclear myopathy
  • Dynamin 2
  • EGFP
  • Fluorescence fluctuation spectroscopy
  • R369W mutation
  • TIRF

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells. / James, Nicholas G.; Digman, Michelle A.; Ross, Justin A.; Barylko, Barbara; Wang, Lei; Li, Jinhui; Chen, Yan; Mueller, Joachim D.; Gratton, Enrico; Albanesi, Joseph P.; Jameson, David M.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1840, No. 1, 2014, p. 315-321.

Research output: Contribution to journalArticle

James, Nicholas G. ; Digman, Michelle A. ; Ross, Justin A. ; Barylko, Barbara ; Wang, Lei ; Li, Jinhui ; Chen, Yan ; Mueller, Joachim D. ; Gratton, Enrico ; Albanesi, Joseph P. ; Jameson, David M. / A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells. In: Biochimica et Biophysica Acta - General Subjects. 2014 ; Vol. 1840, No. 1. pp. 315-321.
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AU - Ross, Justin A.

AU - Barylko, Barbara

AU - Wang, Lei

AU - Li, Jinhui

AU - Chen, Yan

AU - Mueller, Joachim D.

AU - Gratton, Enrico

AU - Albanesi, Joseph P.

AU - Jameson, David M.

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N2 - Background Dynamin 2 (Dyn2) is a ~ 100 kDa GTPase that assembles around the necks of nascent endocytic and Golgi vesicles and catalyzes membrane scission. Mutations in Dyn2 that cause centronuclear myopathy (CNM) have been shown to stabilize Dyn2 polymers against GTP-dependent disassembly in vitro. Precisely timed regulation of assembly and disassembly is believed to be critical for Dyn2 function in membrane vesiculation, and the CNM mutations interfere with this regulation by shifting the equilibrium toward the assembled state. Methods In this study we use two fluorescence fluctuation spectroscopy (FFS) approaches to show that a CNM mutant form of Dyn2 also has a greater propensity to self-assemble in the cytosol and on the plasma membrane of living cells. Results Results obtained using brightness analysis indicate that unassembled wild-type Dyn2 is predominantly tetrameric in the cytosol, although different oligomeric species are observed, depending on the concentration of expressed protein. In contrast, an R369W mutant identified in CNM patients forms higher-order oligomers at concentrations above 1 μM. Investigation of Dyn2-R369W by Total Internal Reflection Fluorescence (TIRF) FFS reveals that this mutant forms larger and more stable clathrin-containing structures on the plasma membrane than wild-type Dyn2. Conclusions and general significance These observations may explain defects in membrane trafficking reported in CNM patient cells and in heterologous systems expressing CNM-associated Dyn2 mutants.

AB - Background Dynamin 2 (Dyn2) is a ~ 100 kDa GTPase that assembles around the necks of nascent endocytic and Golgi vesicles and catalyzes membrane scission. Mutations in Dyn2 that cause centronuclear myopathy (CNM) have been shown to stabilize Dyn2 polymers against GTP-dependent disassembly in vitro. Precisely timed regulation of assembly and disassembly is believed to be critical for Dyn2 function in membrane vesiculation, and the CNM mutations interfere with this regulation by shifting the equilibrium toward the assembled state. Methods In this study we use two fluorescence fluctuation spectroscopy (FFS) approaches to show that a CNM mutant form of Dyn2 also has a greater propensity to self-assemble in the cytosol and on the plasma membrane of living cells. Results Results obtained using brightness analysis indicate that unassembled wild-type Dyn2 is predominantly tetrameric in the cytosol, although different oligomeric species are observed, depending on the concentration of expressed protein. In contrast, an R369W mutant identified in CNM patients forms higher-order oligomers at concentrations above 1 μM. Investigation of Dyn2-R369W by Total Internal Reflection Fluorescence (TIRF) FFS reveals that this mutant forms larger and more stable clathrin-containing structures on the plasma membrane than wild-type Dyn2. Conclusions and general significance These observations may explain defects in membrane trafficking reported in CNM patient cells and in heterologous systems expressing CNM-associated Dyn2 mutants.

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