TY - JOUR
T1 - A Mutation of the Nucleoside Triphosphate Phosphohydrolase I (NPH-I) Gene Confers Sensitivity of Vaccinia Virus to Interferon
AU - Díaz-guerra, Margarita
AU - Kahn, Jeffrey
AU - Esteban, Mariano
PY - 1993/11
Y1 - 1993/11
N2 - The role of vaccinia virus nucleoside triphosphate phosphohydrolase I (NPH-I) in determining the resistance of this virus to α-interferon (IFN) was analyzed by using a well-defined temperature-sensitive mutant (ts36) in the NPH-I gene. Detailed analysis of viral proteins and mRNAs produced in cultured cells treated with or without IFN-α showed a strong inhibition by IFN of the synthesis of late virus polypeptides at the nonpermissive temperature, e.g., 83.8% inhibition for the luciferase reporter gene, which is associated with a decrease in steady-state mRNA levels. Sensitivity of ts36 to IFN was only the consequence of the point mutation in the NPH-1 gene, as shown by characterization of the rescued virus, R36. Our findings demonstrate that functional NPH-1 is required, at least in part, for resistance of vaccinia virus to IFN.
AB - The role of vaccinia virus nucleoside triphosphate phosphohydrolase I (NPH-I) in determining the resistance of this virus to α-interferon (IFN) was analyzed by using a well-defined temperature-sensitive mutant (ts36) in the NPH-I gene. Detailed analysis of viral proteins and mRNAs produced in cultured cells treated with or without IFN-α showed a strong inhibition by IFN of the synthesis of late virus polypeptides at the nonpermissive temperature, e.g., 83.8% inhibition for the luciferase reporter gene, which is associated with a decrease in steady-state mRNA levels. Sensitivity of ts36 to IFN was only the consequence of the point mutation in the NPH-1 gene, as shown by characterization of the rescued virus, R36. Our findings demonstrate that functional NPH-1 is required, at least in part, for resistance of vaccinia virus to IFN.
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U2 - 10.1006/viro.1993.1618
DO - 10.1006/viro.1993.1618
M3 - Article
C2 - 8212591
AN - SCOPUS:0027448566
SN - 0042-6822
VL - 197
SP - 485
EP - 491
JO - Virology
JF - Virology
IS - 1
M1 - 71618
ER -