A myogenic factor from sea urchin embryos capable of programming muscle differentiation in mammalian cells

Judith M. Venuti, Leah Goldberg, Tushar Chakraborty, Eric N. Olson, William H. Klein

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Using the basic helix-loop-helix domain of the myogenic factor myogenin as a probe, we identified a clone from a sea urchin cDNA library with considerable sequence similarity to the vertebrate myogenic factors. This cDNA, sea urchin myogenic factor 1 (SUM-1), transactivated a muscle creatine kinase-chloramphenicol acetyltransferase reporter gene in 10T1/2 fibroblasts to a level comparable to that of the vertebrate myogenic factors. In addition, bacterially expressed β-galactosidase-SUM-1 fusion protein interacted directly with the κE-2 site in the muscle creatine kinase enhancer core as assayed by electrophoretic mobility shift assays. Stably transfected SUM-1 activated the muscle differentiation program and converted 10T1/2 cells from fibroblasts to myotubes. In sea urchin embryos, SUM-1 RNA was not detected before gastrulation. It accumulated to its highest levels during the prism stage when myoblasts were first detected by myosin immunostaining and then diminished as myocytes differentiated. SUM-1 protein was localized in secondary mesenchyme cells when they could first be identified as muscle cells by myosin immunostaining. These results implicate SUM-1 as a regulatory factor involved in the early decision of a pluripotent secondary mesenchyme cell to convert to a myogenic fate. SUM-1 is an example of an invertebrate myogenic factor that is capable of functioning in mammalian cells.

Original languageEnglish (US)
Pages (from-to)6219-6223
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number14
StatePublished - Jul 15 1991

Fingerprint

Sea Urchins
Embryonic Structures
Muscles
MM Form Creatine Kinase
Mesoderm
Myosins
Muscle Cells
Vertebrates
Helix-Loop-Helix Motifs
Fibroblasts
Galactosidases
Myogenin
Gastrulation
Chloramphenicol O-Acetyltransferase
Myoblasts
Skeletal Muscle Fibers
Electrophoretic Mobility Shift Assay
Invertebrates
sea urchin SUM1 protein
Gene Library

Keywords

  • Embryonic development
  • Myogenesis
  • Myogenic conversion
  • Transactivation

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A myogenic factor from sea urchin embryos capable of programming muscle differentiation in mammalian cells. / Venuti, Judith M.; Goldberg, Leah; Chakraborty, Tushar; Olson, Eric N.; Klein, William H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 14, 15.07.1991, p. 6219-6223.

Research output: Contribution to journalArticle

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