A nanodroplet cell processing platform facilitating drug synergy evaluations for anti-cancer treatments

Ching Te Kuo, Jong Yueh Wang, Siang Rong Lu, Yu Sheng Lai, Hsiu Hao Chang, Jer-Tsong Hsieh, Andrew M. Wo, Ping-chi B Chen, Jen Her Lu, Hsinyu Lee

Research output: Contribution to journalArticle

Abstract

Therapeutic drug synergism intervened in cancer treatments has been demonstrated to be more effective than using a single effector. However, it remains inherently challenging, with a limited cell count from tumor samples, to achieve potent personalized drug cocktails. To address the issue above, we herein present a nanodroplet cell processing platform. The platform incorporates an automatic nanodroplet dispenser with cell array ParaStamp chips, which were fabricated by a new wax stamping approach derived from laser direct writing. Such approach enables not only the on-demand de-wetting with hydrophobic wax films on substrates but also the mask-less fabrication of non-planar microstructures (i.e. no photolithography process). The ParaStamp chip was pre-occupied with anti-cancer drugs and their associate mixtures, enabling for the spatially addressable screening of optimal drug combinations simultaneously. Each droplet with a critical volume of 200 nl containing with 100 cells was utilized. Results revealed that the optimal combination reduces approximate 28-folds of conducted doses compared with single drugs. Tumor inhibition with the optimally selected drug combination was further confirmed by using PC-3 tumor-bearing mouse models. Together, the nanodroplet cell processing platform could therefore offer new opportunities to power the personalized cancer medicine at early-stage drug screening and discovery.

Original languageEnglish (US)
Article number10120
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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