A new application of charged aerosol detection in liquid chromatography for the simultaneous determination of polar and less polar ginsenosides in ginseng products

Shaodong Jia, Jing Li, Nozimakhonim Yunusova, Jeong Hill Park, Sung Won Kwon, Jeongmi Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Introduction Conventional liquid chromatographic methods coupled with ultraviolet or evaporative light scattering detection are not sensitive enough to determine both polar and less polar ginsenosides at low concentrations. Objective To establish a liquid chromatography-charged aerosol detection method for the simultaneous determination of polar and less polar ginsenosides in a variety of ginseng products Methods Fourteen polar and less polar ginsenosides were extracted and concentrated by solid phase extraction. These were subsequently baseline-separated on a conventional reversed-phase C 18-column (250 mm × 4.6 mm, 5 μm) with a simple mobile phase consisting of water and acetonitrile. Components were then detected by means of charged aerosol detection. Results The method developed allowed the simultaneous determination of six polar ginsenosides (Rg1, Re, Rb1, Rc, Rb, Rd) and eight less polar ginsenosides (Rg6, F4, Rk3, Rh4, Rg3(S), Rg 3(R), Rk1, Rg5) in a single chromatographic run. Further, the method was linear (R2 > 0.99), accurate (relative recoveries, 90-112%), and precise (intraday RSD < 5.7% and interday RSD < 10.6%) within the concentration range tested. The method sensitivity was measured in terms of the limit of detection, which ranged from 0.5 to 4.0 μg/mL. Conclusion Concentrations of 14 ginsenosides were determined simultaneously in one homemade red ginseng and 13 commercial ginseng products of different types (liquid and solid samples), and results showed that ginsenoside content varied significantly among the samples tested. The method developed could serve as a useful analytical tool for the quality control of ginseng products.

Original languageEnglish (US)
Pages (from-to)374-380
Number of pages7
JournalPhytochemical Analysis
Volume24
Issue number4
DOIs
StatePublished - Jul 2013

Fingerprint

Ginsenosides
Panax
Liquid chromatography
aerosols
Aerosols
Liquid Chromatography
liquid chromatography
methodology
Liquids
Light scattering
Quality control
liquids
Solid Phase Extraction
light scattering
solid phase extraction
ginsenosides
Quality Control
Recovery
quality control
Limit of Detection

Keywords

  • Charged aerosol detection
  • ginseng products
  • less polar ginsenosides
  • liquid chromatography
  • polar ginsenosides
  • quality control

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Drug Discovery
  • Food Science
  • Biochemistry
  • Analytical Chemistry
  • Plant Science

Cite this

A new application of charged aerosol detection in liquid chromatography for the simultaneous determination of polar and less polar ginsenosides in ginseng products. / Jia, Shaodong; Li, Jing; Yunusova, Nozimakhonim; Park, Jeong Hill; Kwon, Sung Won; Lee, Jeongmi.

In: Phytochemical Analysis, Vol. 24, No. 4, 07.2013, p. 374-380.

Research output: Contribution to journalArticle

Jia, Shaodong ; Li, Jing ; Yunusova, Nozimakhonim ; Park, Jeong Hill ; Kwon, Sung Won ; Lee, Jeongmi. / A new application of charged aerosol detection in liquid chromatography for the simultaneous determination of polar and less polar ginsenosides in ginseng products. In: Phytochemical Analysis. 2013 ; Vol. 24, No. 4. pp. 374-380.
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abstract = "Introduction Conventional liquid chromatographic methods coupled with ultraviolet or evaporative light scattering detection are not sensitive enough to determine both polar and less polar ginsenosides at low concentrations. Objective To establish a liquid chromatography-charged aerosol detection method for the simultaneous determination of polar and less polar ginsenosides in a variety of ginseng products Methods Fourteen polar and less polar ginsenosides were extracted and concentrated by solid phase extraction. These were subsequently baseline-separated on a conventional reversed-phase C 18-column (250 mm × 4.6 mm, 5 μm) with a simple mobile phase consisting of water and acetonitrile. Components were then detected by means of charged aerosol detection. Results The method developed allowed the simultaneous determination of six polar ginsenosides (Rg1, Re, Rb1, Rc, Rb, Rd) and eight less polar ginsenosides (Rg6, F4, Rk3, Rh4, Rg3(S), Rg 3(R), Rk1, Rg5) in a single chromatographic run. Further, the method was linear (R2 > 0.99), accurate (relative recoveries, 90-112{\%}), and precise (intraday RSD < 5.7{\%} and interday RSD < 10.6{\%}) within the concentration range tested. The method sensitivity was measured in terms of the limit of detection, which ranged from 0.5 to 4.0 μg/mL. Conclusion Concentrations of 14 ginsenosides were determined simultaneously in one homemade red ginseng and 13 commercial ginseng products of different types (liquid and solid samples), and results showed that ginsenoside content varied significantly among the samples tested. The method developed could serve as a useful analytical tool for the quality control of ginseng products.",
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AU - Lee, Jeongmi

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N2 - Introduction Conventional liquid chromatographic methods coupled with ultraviolet or evaporative light scattering detection are not sensitive enough to determine both polar and less polar ginsenosides at low concentrations. Objective To establish a liquid chromatography-charged aerosol detection method for the simultaneous determination of polar and less polar ginsenosides in a variety of ginseng products Methods Fourteen polar and less polar ginsenosides were extracted and concentrated by solid phase extraction. These were subsequently baseline-separated on a conventional reversed-phase C 18-column (250 mm × 4.6 mm, 5 μm) with a simple mobile phase consisting of water and acetonitrile. Components were then detected by means of charged aerosol detection. Results The method developed allowed the simultaneous determination of six polar ginsenosides (Rg1, Re, Rb1, Rc, Rb, Rd) and eight less polar ginsenosides (Rg6, F4, Rk3, Rh4, Rg3(S), Rg 3(R), Rk1, Rg5) in a single chromatographic run. Further, the method was linear (R2 > 0.99), accurate (relative recoveries, 90-112%), and precise (intraday RSD < 5.7% and interday RSD < 10.6%) within the concentration range tested. The method sensitivity was measured in terms of the limit of detection, which ranged from 0.5 to 4.0 μg/mL. Conclusion Concentrations of 14 ginsenosides were determined simultaneously in one homemade red ginseng and 13 commercial ginseng products of different types (liquid and solid samples), and results showed that ginsenoside content varied significantly among the samples tested. The method developed could serve as a useful analytical tool for the quality control of ginseng products.

AB - Introduction Conventional liquid chromatographic methods coupled with ultraviolet or evaporative light scattering detection are not sensitive enough to determine both polar and less polar ginsenosides at low concentrations. Objective To establish a liquid chromatography-charged aerosol detection method for the simultaneous determination of polar and less polar ginsenosides in a variety of ginseng products Methods Fourteen polar and less polar ginsenosides were extracted and concentrated by solid phase extraction. These were subsequently baseline-separated on a conventional reversed-phase C 18-column (250 mm × 4.6 mm, 5 μm) with a simple mobile phase consisting of water and acetonitrile. Components were then detected by means of charged aerosol detection. Results The method developed allowed the simultaneous determination of six polar ginsenosides (Rg1, Re, Rb1, Rc, Rb, Rd) and eight less polar ginsenosides (Rg6, F4, Rk3, Rh4, Rg3(S), Rg 3(R), Rk1, Rg5) in a single chromatographic run. Further, the method was linear (R2 > 0.99), accurate (relative recoveries, 90-112%), and precise (intraday RSD < 5.7% and interday RSD < 10.6%) within the concentration range tested. The method sensitivity was measured in terms of the limit of detection, which ranged from 0.5 to 4.0 μg/mL. Conclusion Concentrations of 14 ginsenosides were determined simultaneously in one homemade red ginseng and 13 commercial ginseng products of different types (liquid and solid samples), and results showed that ginsenoside content varied significantly among the samples tested. The method developed could serve as a useful analytical tool for the quality control of ginseng products.

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KW - less polar ginsenosides

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KW - polar ginsenosides

KW - quality control

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