A new class of scorpion toxin binding sites related to an A-type K+ channel

Pharmacological characterization and localization in rat brain

Hélène Vacher, Régine Romi-Lebrun, Christiane Mourre, Bruno Lebrun, Said Kourrich, Frédérique Masméjean, Terumi Nakajima, Christian Legros, Marcel Crest, Pierre E. Bougis, Marie France Martin-Eauclaire

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A new scorpion toxin (3751.8 Da) was isolated from the Buthus martensi venom, sequenced and chemically synthesized (sBmTXS). The A-type current of striatum neurons in culture completely disappeared when 1 μM BmTX3 was applied (Kd = 54 nM), whereas the sustained K+ current was unaffected. 125I-sBmTX3 specifically bound to rat brain synaptosomes (maximum binding = 14 fmol mg-1 of protein, Kd = 0.21 nM). A panel of toxins yet described as specific ligands for K+ channels were unable to compete with 125I-sBmTX3. A high density of 125I-sBmTX3 binding sites was found in the striatum, hippocampus, superior colliculus, and cerebellum in the adult rat brain.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalFEBS Letters
Volume501
Issue number1-3
StatePublished - Jul 20 2001

Fingerprint

Scorpions
Rats
Brain
Binding Sites
Pharmacology
Superior Colliculi
Synaptosomes
Venoms
Cerebellum
Neurons
Hippocampus
Ligands
Proteins

Keywords

  • A-type K current
  • Distribution in rat brain
  • Scorpion toxin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Vacher, H., Romi-Lebrun, R., Mourre, C., Lebrun, B., Kourrich, S., Masméjean, F., ... Martin-Eauclaire, M. F. (2001). A new class of scorpion toxin binding sites related to an A-type K+ channel: Pharmacological characterization and localization in rat brain. FEBS Letters, 501(1-3), 31-36.

A new class of scorpion toxin binding sites related to an A-type K+ channel : Pharmacological characterization and localization in rat brain. / Vacher, Hélène; Romi-Lebrun, Régine; Mourre, Christiane; Lebrun, Bruno; Kourrich, Said; Masméjean, Frédérique; Nakajima, Terumi; Legros, Christian; Crest, Marcel; Bougis, Pierre E.; Martin-Eauclaire, Marie France.

In: FEBS Letters, Vol. 501, No. 1-3, 20.07.2001, p. 31-36.

Research output: Contribution to journalArticle

Vacher, H, Romi-Lebrun, R, Mourre, C, Lebrun, B, Kourrich, S, Masméjean, F, Nakajima, T, Legros, C, Crest, M, Bougis, PE & Martin-Eauclaire, MF 2001, 'A new class of scorpion toxin binding sites related to an A-type K+ channel: Pharmacological characterization and localization in rat brain', FEBS Letters, vol. 501, no. 1-3, pp. 31-36.
Vacher, Hélène ; Romi-Lebrun, Régine ; Mourre, Christiane ; Lebrun, Bruno ; Kourrich, Said ; Masméjean, Frédérique ; Nakajima, Terumi ; Legros, Christian ; Crest, Marcel ; Bougis, Pierre E. ; Martin-Eauclaire, Marie France. / A new class of scorpion toxin binding sites related to an A-type K+ channel : Pharmacological characterization and localization in rat brain. In: FEBS Letters. 2001 ; Vol. 501, No. 1-3. pp. 31-36.
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