A new clonal strain of rat pituitary tumour cells: A model for non-regulated secretion of prolactin

M. J. Reymond, D. D. Nansel, G. H. Burrows, W. B. Neaves, J. C. Porter

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A new clonal strain of Prl-secreting cells derived from the transplantable rat pituitary tumour, 73 15a, has been established in culture. The cells of this strain, designed 235-1, have a highly developed Golgi complex, an extensive rough endoplasmic reticulum, and a few small but no large dense-core granules. When inoculated into athymic mice and rats of the Buffalo strain, the 235-1 cells produce tumours, and the host animals have hypertrophied mammary glands that produce milk, indicating the Prl secreted by these cells has mammotrophic activity. In monolayer culture, the doubling time of 235-1 cells is 3 1 ± 1 h (mean ± SE). The cells secrete Prl, a trace quantity of GH, but no LH, FSH, TSH, ACTH, or α-MSH. Prl is released at a rate of 257 ± 12 fg per h per cell. The cellular content of Prl is 424 ± 23 fg per cell. Prl secretion by 235-1 cells is not affected by dopaminergic agonists and antagonists. TRH, or oestradiol-17β but is inhibited in the presence of EGTA or monensin, an ionophore that is believed to act at the level of the Golgi complex. The subcellular distribution of Prl in 235-1 cells is different from that in rat pituitary cells. In 235-1 cells, Prl is associated not with a single set of dense particles as it is in pituitary cells but with 2 sets of subcellular particles, of which 1 set cosedimented with particles having lysosomal enzyme activity. These findings suggest that Prl secretion by 235-1 cells involves secretory pathways that are different from those seen in normal lactotrophs.

Original languageEnglish (US)
Pages (from-to)459-470
Number of pages12
JournalActa Endocrinologica
Volume106
Issue number4
StatePublished - 1984

Fingerprint

Pituitary Neoplasms
Prolactin
Golgi Apparatus
Lactotrophs
Nude Rats
Melanocyte-Stimulating Hormones
Monensin
Dopamine Antagonists
Rough Endoplasmic Reticulum
Secretory Pathway
Egtazic Acid
Buffaloes
Dopamine Agonists
Ionophores
Human Mammary Glands
Nude Mice
Adrenocorticotropic Hormone
Estradiol
Milk

ASJC Scopus subject areas

  • Endocrinology

Cite this

Reymond, M. J., Nansel, D. D., Burrows, G. H., Neaves, W. B., & Porter, J. C. (1984). A new clonal strain of rat pituitary tumour cells: A model for non-regulated secretion of prolactin. Acta Endocrinologica, 106(4), 459-470.

A new clonal strain of rat pituitary tumour cells : A model for non-regulated secretion of prolactin. / Reymond, M. J.; Nansel, D. D.; Burrows, G. H.; Neaves, W. B.; Porter, J. C.

In: Acta Endocrinologica, Vol. 106, No. 4, 1984, p. 459-470.

Research output: Contribution to journalArticle

Reymond, MJ, Nansel, DD, Burrows, GH, Neaves, WB & Porter, JC 1984, 'A new clonal strain of rat pituitary tumour cells: A model for non-regulated secretion of prolactin', Acta Endocrinologica, vol. 106, no. 4, pp. 459-470.
Reymond MJ, Nansel DD, Burrows GH, Neaves WB, Porter JC. A new clonal strain of rat pituitary tumour cells: A model for non-regulated secretion of prolactin. Acta Endocrinologica. 1984;106(4):459-470.
Reymond, M. J. ; Nansel, D. D. ; Burrows, G. H. ; Neaves, W. B. ; Porter, J. C. / A new clonal strain of rat pituitary tumour cells : A model for non-regulated secretion of prolactin. In: Acta Endocrinologica. 1984 ; Vol. 106, No. 4. pp. 459-470.
@article{251533721c5a4d80bc39cd8b042b586d,
title = "A new clonal strain of rat pituitary tumour cells: A model for non-regulated secretion of prolactin",
abstract = "A new clonal strain of Prl-secreting cells derived from the transplantable rat pituitary tumour, 73 15a, has been established in culture. The cells of this strain, designed 235-1, have a highly developed Golgi complex, an extensive rough endoplasmic reticulum, and a few small but no large dense-core granules. When inoculated into athymic mice and rats of the Buffalo strain, the 235-1 cells produce tumours, and the host animals have hypertrophied mammary glands that produce milk, indicating the Prl secreted by these cells has mammotrophic activity. In monolayer culture, the doubling time of 235-1 cells is 3 1 ± 1 h (mean ± SE). The cells secrete Prl, a trace quantity of GH, but no LH, FSH, TSH, ACTH, or α-MSH. Prl is released at a rate of 257 ± 12 fg per h per cell. The cellular content of Prl is 424 ± 23 fg per cell. Prl secretion by 235-1 cells is not affected by dopaminergic agonists and antagonists. TRH, or oestradiol-17β but is inhibited in the presence of EGTA or monensin, an ionophore that is believed to act at the level of the Golgi complex. The subcellular distribution of Prl in 235-1 cells is different from that in rat pituitary cells. In 235-1 cells, Prl is associated not with a single set of dense particles as it is in pituitary cells but with 2 sets of subcellular particles, of which 1 set cosedimented with particles having lysosomal enzyme activity. These findings suggest that Prl secretion by 235-1 cells involves secretory pathways that are different from those seen in normal lactotrophs.",
author = "Reymond, {M. J.} and Nansel, {D. D.} and Burrows, {G. H.} and Neaves, {W. B.} and Porter, {J. C.}",
year = "1984",
language = "English (US)",
volume = "106",
pages = "459--470",
journal = "European Journal of Endocrinology",
issn = "0804-4643",
publisher = "BioScientifica Ltd.",
number = "4",

}

TY - JOUR

T1 - A new clonal strain of rat pituitary tumour cells

T2 - A model for non-regulated secretion of prolactin

AU - Reymond, M. J.

AU - Nansel, D. D.

AU - Burrows, G. H.

AU - Neaves, W. B.

AU - Porter, J. C.

PY - 1984

Y1 - 1984

N2 - A new clonal strain of Prl-secreting cells derived from the transplantable rat pituitary tumour, 73 15a, has been established in culture. The cells of this strain, designed 235-1, have a highly developed Golgi complex, an extensive rough endoplasmic reticulum, and a few small but no large dense-core granules. When inoculated into athymic mice and rats of the Buffalo strain, the 235-1 cells produce tumours, and the host animals have hypertrophied mammary glands that produce milk, indicating the Prl secreted by these cells has mammotrophic activity. In monolayer culture, the doubling time of 235-1 cells is 3 1 ± 1 h (mean ± SE). The cells secrete Prl, a trace quantity of GH, but no LH, FSH, TSH, ACTH, or α-MSH. Prl is released at a rate of 257 ± 12 fg per h per cell. The cellular content of Prl is 424 ± 23 fg per cell. Prl secretion by 235-1 cells is not affected by dopaminergic agonists and antagonists. TRH, or oestradiol-17β but is inhibited in the presence of EGTA or monensin, an ionophore that is believed to act at the level of the Golgi complex. The subcellular distribution of Prl in 235-1 cells is different from that in rat pituitary cells. In 235-1 cells, Prl is associated not with a single set of dense particles as it is in pituitary cells but with 2 sets of subcellular particles, of which 1 set cosedimented with particles having lysosomal enzyme activity. These findings suggest that Prl secretion by 235-1 cells involves secretory pathways that are different from those seen in normal lactotrophs.

AB - A new clonal strain of Prl-secreting cells derived from the transplantable rat pituitary tumour, 73 15a, has been established in culture. The cells of this strain, designed 235-1, have a highly developed Golgi complex, an extensive rough endoplasmic reticulum, and a few small but no large dense-core granules. When inoculated into athymic mice and rats of the Buffalo strain, the 235-1 cells produce tumours, and the host animals have hypertrophied mammary glands that produce milk, indicating the Prl secreted by these cells has mammotrophic activity. In monolayer culture, the doubling time of 235-1 cells is 3 1 ± 1 h (mean ± SE). The cells secrete Prl, a trace quantity of GH, but no LH, FSH, TSH, ACTH, or α-MSH. Prl is released at a rate of 257 ± 12 fg per h per cell. The cellular content of Prl is 424 ± 23 fg per cell. Prl secretion by 235-1 cells is not affected by dopaminergic agonists and antagonists. TRH, or oestradiol-17β but is inhibited in the presence of EGTA or monensin, an ionophore that is believed to act at the level of the Golgi complex. The subcellular distribution of Prl in 235-1 cells is different from that in rat pituitary cells. In 235-1 cells, Prl is associated not with a single set of dense particles as it is in pituitary cells but with 2 sets of subcellular particles, of which 1 set cosedimented with particles having lysosomal enzyme activity. These findings suggest that Prl secretion by 235-1 cells involves secretory pathways that are different from those seen in normal lactotrophs.

UR - http://www.scopus.com/inward/record.url?scp=0021193880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021193880&partnerID=8YFLogxK

M3 - Article

C2 - 6433612

AN - SCOPUS:0021193880

VL - 106

SP - 459

EP - 470

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

SN - 0804-4643

IS - 4

ER -