A new immunochemical assay for biotin

Le Phuc Thuy, L. Sweetman, W. L. Nyhan

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 /μl of plasma or 5-10 μl of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 ± 0.67 nmol/l and 49.1 ± 35.7 μmol/mol creatinine, respectively.

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalClinica Chimica Acta
Volume202
Issue number3
DOIs
StatePublished - Oct 31 1991
Externally publishedYes

Keywords

  • Biotin
  • Immunochemical assay

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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