A new immunochemical assay for biotin

Le Phuc Thuy; L. Sweetman; W. L. Nyhan

doi:10.1016/0009-8981(91)90049-I

A new immunochemical assay for biotin

Le Phuc Thuy, L. Sweetman, W. L. Nyhan

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [³H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 /μl of plasma or 5-10 μl of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 ± 0.67 nmol/l and 49.1 ± 35.7 μmol/mol creatinine, respectively.

Original language	English (US)
Pages (from-to)	191-197
Number of pages	7
Journal	Clinica Chimica Acta
Volume	202
Issue number	3
DOIs	https://doi.org/10.1016/0009-8981(91)90049-I
State	Published - Oct 31 1991
Externally published	Yes

Keywords

Biotin
Immunochemical assay

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

Access to Document

10.1016/0009-8981(91)90049-I

Cite this

@article{d0da1990f01745f4acc0027b263d2926,

title = "A new immunochemical assay for biotin",

abstract = "A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 /μl of plasma or 5-10 μl of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 ± 0.67 nmol/l and 49.1 ± 35.7 μmol/mol creatinine, respectively.",

keywords = "Biotin, Immunochemical assay",

author = "Thuy, {Le Phuc} and L. Sweetman and Nyhan, {W. L.}",

year = "1991",

month = oct,

day = "31",

doi = "10.1016/0009-8981(91)90049-I",

language = "English (US)",

volume = "202",

pages = "191--197",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - A new immunochemical assay for biotin

AU - Thuy, Le Phuc

AU - Sweetman, L.

AU - Nyhan, W. L.

PY - 1991/10/31

Y1 - 1991/10/31

N2 - A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 /μl of plasma or 5-10 μl of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 ± 0.67 nmol/l and 49.1 ± 35.7 μmol/mol creatinine, respectively.

AB - A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 /μl of plasma or 5-10 μl of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 ± 0.67 nmol/l and 49.1 ± 35.7 μmol/mol creatinine, respectively.

KW - Biotin

KW - Immunochemical assay

UR - http://www.scopus.com/inward/record.url?scp=0025947098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025947098&partnerID=8YFLogxK

U2 - 10.1016/0009-8981(91)90049-I

DO - 10.1016/0009-8981(91)90049-I

M3 - Article

C2 - 1814646

AN - SCOPUS:0025947098

SN - 0009-8981

VL - 202

SP - 191

EP - 197

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 3

ER -

A new immunochemical assay for biotin

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this