A new mouse insertional mutation that causes sensorineural deafness and vestibular defects

K. N. Alagramam, H. Y. Kwon, N. L A Cacheiro, L. Stubbs, C. G. Wright, L. C. Erway, Richard P. Woychik

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This article describes a new recessive insertional mutation in the transgenic line TgN2742Rpw that causes deafness and circling behavior in mice. Histologic analysis revealed virtually complete loss of the cochlear neuroepithelium (the organ of Corti) in adult mutant mice. In association with the neuroepithelial changes, there is a dramatic reduction of the cochlear nerve supply. Adult mutants also show morphological defects of the vestibular apparatus, including degeneration of the saccular neuroepithelium and occasional malformation of utricular otoconia. Audiometric evaluations demonstrated that the mice displaying the circling phenotype are completely deaf. Molecular analysis of this mutant line revealed that the transgenic insertion occurred without creating a large deletion of the host DNA sequences. The mutant locus was mapped to a region on mouse chromosome 10, where other spontaneous, recessive mutations causing deafness in mice have been mapped.

Original languageEnglish (US)
Pages (from-to)1691-1699
Number of pages9
JournalGenetics
Volume152
Issue number4
StatePublished - Aug 1999

Fingerprint

Deafness
Mutation
Labyrinth Vestibule
Otolithic Membrane
Organ of Corti
Chromosomes, Human, Pair 10
Cochlear Nerve
Cochlea
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Alagramam, K. N., Kwon, H. Y., Cacheiro, N. L. A., Stubbs, L., Wright, C. G., Erway, L. C., & Woychik, R. P. (1999). A new mouse insertional mutation that causes sensorineural deafness and vestibular defects. Genetics, 152(4), 1691-1699.

A new mouse insertional mutation that causes sensorineural deafness and vestibular defects. / Alagramam, K. N.; Kwon, H. Y.; Cacheiro, N. L A; Stubbs, L.; Wright, C. G.; Erway, L. C.; Woychik, Richard P.

In: Genetics, Vol. 152, No. 4, 08.1999, p. 1691-1699.

Research output: Contribution to journalArticle

Alagramam, KN, Kwon, HY, Cacheiro, NLA, Stubbs, L, Wright, CG, Erway, LC & Woychik, RP 1999, 'A new mouse insertional mutation that causes sensorineural deafness and vestibular defects', Genetics, vol. 152, no. 4, pp. 1691-1699.
Alagramam KN, Kwon HY, Cacheiro NLA, Stubbs L, Wright CG, Erway LC et al. A new mouse insertional mutation that causes sensorineural deafness and vestibular defects. Genetics. 1999 Aug;152(4):1691-1699.
Alagramam, K. N. ; Kwon, H. Y. ; Cacheiro, N. L A ; Stubbs, L. ; Wright, C. G. ; Erway, L. C. ; Woychik, Richard P. / A new mouse insertional mutation that causes sensorineural deafness and vestibular defects. In: Genetics. 1999 ; Vol. 152, No. 4. pp. 1691-1699.
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