A new transient activator of phosphofructokinase during initiation of rapid glycolysis in brain

Susumu Ogushi, J. W Randy Lawson, Geoffrey P. Dobson, Richard L. Veech, Kosaku Uyeda

Research output: Contribution to journalArticle

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Abstract

The tissue contents of previously known allosteric effectors of brain phosphofructokinase (EC 2.7.1.11) (PFK) and the kinetic behavior of isolated PFK were investigated during the initiation of rapid glycolytic flux in freeze-blown rat brain. Comparing 0- with 5-s brains revealed that there was a 4-fold drop in total tissue content of Fru-6-P and a 5.6-fold increase in Fru-1,6-P2 consistent with activation of PFK. Additionally, analysis of brain content showed a 15-fold increase in AMP, a 3-fold decrease in ATP, a 3-fold decrease in Pi, and a 1.6-fold increase in NH+4. There was no change in Fru-2,6-P2, H+, citrate, or Glc-1,6-P2 or the kinetic profiles of isolated PFK for ATP inhibition or Fru-2,6-P2 activation. We concluded that the observed change in PFK activity could be accounted for only partially by changes in the concentrations of adenine nucleotides and other known effectors. High performance liquid chromatography fractions of extracts obtained from 5-s brains showed the activator with a mobility identical to ribose 1,5-P2 and gave 2 nmol/g (wet weight) at 0 s, 10 nmol/g at 5 s, and 2 nmol/g at 20 s. Assay of PFK in the presence of effectors determined to be in tissue at 5 s showed that addition of 10 nmol/ml ribose 1,5-P2 gave a 4-fold activation of PFK. Based on the rapidity of its formation, its potency of activation, and its similarity in chemical properties to authentic ribose 1,5-P2, we conclude that ribose 1,5-P2 served as the initial activator of PFK in brain.

Original languageEnglish (US)
Pages (from-to)10943-10949
Number of pages7
JournalJournal of Biological Chemistry
Volume265
Issue number19
StatePublished - Jul 5 1990

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Glycolysis
Brain
Ribose
Chemical activation
Tissue
Adenosine Triphosphate
Phosphofructokinases
Kinetics
Adenine Nucleotides
High performance liquid chromatography
Adenosine Monophosphate
Citric Acid
Chemical properties
fructose 2,6-diphosphate
Rats
Assays
High Pressure Liquid Chromatography
Fluxes
Weights and Measures

ASJC Scopus subject areas

  • Biochemistry

Cite this

A new transient activator of phosphofructokinase during initiation of rapid glycolysis in brain. / Ogushi, Susumu; Lawson, J. W Randy; Dobson, Geoffrey P.; Veech, Richard L.; Uyeda, Kosaku.

In: Journal of Biological Chemistry, Vol. 265, No. 19, 05.07.1990, p. 10943-10949.

Research output: Contribution to journalArticle

Ogushi, S, Lawson, JWR, Dobson, GP, Veech, RL & Uyeda, K 1990, 'A new transient activator of phosphofructokinase during initiation of rapid glycolysis in brain', Journal of Biological Chemistry, vol. 265, no. 19, pp. 10943-10949.
Ogushi, Susumu ; Lawson, J. W Randy ; Dobson, Geoffrey P. ; Veech, Richard L. ; Uyeda, Kosaku. / A new transient activator of phosphofructokinase during initiation of rapid glycolysis in brain. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 19. pp. 10943-10949.
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