A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment

Hao Luo, Doug W. Chan, Tao Yang, Maria Rodriguez, Benjamin Ping Chi Chen, Mei Leng, Jung Jung Mu, David Chen, Zhou Songyang, Yi Wang, Jun Qin

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

DNA single-strand break repair (SSBR) is important for maintaining genome stability and homeostasis. The current SSBR model derived from an in vitro-reconstituted reaction suggests that the SSBR complex mediated by X-ray repair cross-complementing protein 1 (XRCC1) is assembled sequentially at the site of damage. In this study, we provide biochemical data to demonstrate that two preformed XRCC1 protein complexes exist in cycling HeLa cells. One complex contains known enzymes that are important for SSBR, including DNA ligase 3 (DNL3), polynucleotide kinase 3′-phosphatase, and polymerase β; the other is a new complex that contains DNL3 and the ataxia with oculomotor apraxia type 1 (AOA) gene product aprataxin. We report the characterization of the new XRCC1 complex. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on S518, T519, and T523 largely determines aprataxin binding to XRCC1 though its FHA domain. An acute loss of aprataxin by small interfering RNA renders HeLa cells sensitive to methyl methanesulfonate treatment by a mechanism of shortened half-life of XRCC1. Thus, aprataxin plays a role to maintain the steady-state protein level of XRCC1. Collectively, these data provide insights into the SSBR molecular machinery in the cell and point to the involvement of aprataxin in SSBR, thus linking SSBR to the neurological disease AOA.

Original languageEnglish (US)
Pages (from-to)8356-8365
Number of pages10
JournalMolecular and Cellular Biology
Volume24
Issue number19
DOIs
StatePublished - Oct 2004

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Methyl Methanesulfonate
DNA Ligases
HeLa Cells
Polynucleotide 5'-Hydroxyl-Kinase
Single-Stranded DNA Breaks
X-ray repair cross complementing protein 1
Genomic Instability
Phosphoric Monoester Hydrolases
Small Interfering RNA
Half-Life
Proteins
Homeostasis
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment. / Luo, Hao; Chan, Doug W.; Yang, Tao; Rodriguez, Maria; Chen, Benjamin Ping Chi; Leng, Mei; Mu, Jung Jung; Chen, David; Songyang, Zhou; Wang, Yi; Qin, Jun.

In: Molecular and Cellular Biology, Vol. 24, No. 19, 10.2004, p. 8356-8365.

Research output: Contribution to journalArticle

Luo, H, Chan, DW, Yang, T, Rodriguez, M, Chen, BPC, Leng, M, Mu, JJ, Chen, D, Songyang, Z, Wang, Y & Qin, J 2004, 'A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment', Molecular and Cellular Biology, vol. 24, no. 19, pp. 8356-8365. https://doi.org/10.1128/MCB.24.19.8356-8365.2004
Luo, Hao ; Chan, Doug W. ; Yang, Tao ; Rodriguez, Maria ; Chen, Benjamin Ping Chi ; Leng, Mei ; Mu, Jung Jung ; Chen, David ; Songyang, Zhou ; Wang, Yi ; Qin, Jun. / A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 19. pp. 8356-8365.
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