A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells

Saipraveen Srinivasan, Christoph J. Burckhardt, Madhura Bhave, Zhiming Chen, Ping Hung Chen, Xinxin Wang, Gaudenz Danuser, Sandra L. Schmid

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Dynamin Guanosine Triphosphate hydrolases (GTPases) are best studied for their role in the terminal membrane fission process of clathrin-mediated endocytosis (CME), but they have also been proposed to regulate earlier stages of CME. Although highly enriched in neurons, dynamin-1 (Dyn1) is, in fact, widely expressed along with Dyn2 but inactivated in non-neuronal cells via phosphorylation by glycogen synthase kinase-3 beta (GSK3β) kinase. Here, we study the differential, isoform-specific functions of Dyn1 and Dyn2 as regulators of CME. Endogenously expressed Dyn1 and Dyn2 were fluorescently tagged either separately or together in two cell lines with contrasting Dyn1 expression levels. By quantitative live cell dual- and triple-channel total internal reflection fluorescence microscopy, we find that Dyn2 is more efficiently recruited to clathrin-coated pits (CCPs) than Dyn1, and that Dyn2 but not Dyn1 exhibits a pronounced burst of assembly, presumably into supramolecular collar-like structures that drive membrane scission and clathrin-coated vesicle (CCV) formation. Activation of Dyn1 by acute inhibition of GSK3β results in more rapid endocytosis of transferrin receptors, increased rates of CCP initiation, and decreased CCP lifetimes but did not significantly affect the extent of Dyn1 recruitment to CCPs. Thus, activated Dyn1 can regulate early stages of CME that occur well upstream of fission, even when present at low, substoichiometric levels relative to Dyn2. Under physiological conditions, Dyn1 is activated downstream of epidermal growth factor receptor (EGFR) signaling to alter CCP dynamics. We identify sorting nexin 9 (SNX9) as a preferred binding partner to activated Dyn1 that is partially required for Dyn1-dependent effects on early stages of CCP maturation. Together, we decouple regulatory and scission functions of dynamins and report a scission-independent, isoform-specific regulatory role for Dyn1 in CME.

Original languageEnglish (US)
Article numbere2005377
JournalPLoS Biology
Volume16
Issue number4
DOIs
StatePublished - Apr 18 2018

Fingerprint

Dynamin I
clathrin
Clathrin
endocytosis
Endocytosis
cells
Dynamins
Glycogen Synthase Kinase 3
dynamins
Sorting Nexins
Protein Isoforms
Clathrin-Coated Vesicles
guanosine triphosphate
Membranes
Phosphorylation
Transferrin Receptors
coated vesicles
Fluorescence microscopy
Hydrolases

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells. / Srinivasan, Saipraveen; Burckhardt, Christoph J.; Bhave, Madhura; Chen, Zhiming; Chen, Ping Hung; Wang, Xinxin; Danuser, Gaudenz; Schmid, Sandra L.

In: PLoS Biology, Vol. 16, No. 4, e2005377, 18.04.2018.

Research output: Contribution to journalArticle

Srinivasan, Saipraveen ; Burckhardt, Christoph J. ; Bhave, Madhura ; Chen, Zhiming ; Chen, Ping Hung ; Wang, Xinxin ; Danuser, Gaudenz ; Schmid, Sandra L. / A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells. In: PLoS Biology. 2018 ; Vol. 16, No. 4.
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AU - Chen, Zhiming

AU - Chen, Ping Hung

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AU - Danuser, Gaudenz

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