A nonclassical bHLH-Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling

Kei Hori, Justyna Cholewa-Waclaw, Yuji Nakada, Stacey M. Glasgow, Toshihiko Masui, R. Michael Henke, Hendrik Wildner, Benedetta Martarelli, Thomas M. Beres, Jonathan A. Epstein, Mark A. Magnuson, Raymond J. MacDonald, Carmen Birchmeier, Jane E. Johnson

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Neural networks are balanced by inhibitory and excitatory neuronal activity. The formation of these networks is initially generated through neuronal subtype specification controlled by transcription factors. The basic helix-loop-helix (bHLH) transcription factor Ptf1a is essential for the generation of GABAergic inhibitory neurons in the dorsal spinal cord, cerebellum, and retina. The transcription factor Rbpj is a transducer of the Notch signaling pathway that functions to maintain neural progenitor cells. Here we demonstrate Ptf1a and Rbpj interact in a complex that is required in vivo for specification of the GABAergic neurons, a function that cannot be substituted by the classical form of the bHLH heterodimer with E-protein or Notch signaling through Rbpj. We show that a mutant form of Ptf1a without the ability to bind Rbpj, while retaining its ability to interact with E-protein, is incapable of inducing GABAergic (Pax2)- and suppressing glutamatergic (Tlx3)-expressing cells in the chick and mouse neural tube. Moreover, we use an Rbpj conditional mutation to demonstrate that Rbpj function is essential for GABAergic specification, and that this function is independent of the Notch signaling pathway. Together, these findings demonstrate the requirement for a Ptf1a-Rbpj complex in controlling the balanced formation of inhibitory and excitatory neurons in the developing spinal cord, and point to a novel Notch-independent function for Rbpj in nervous system development.

Original languageEnglish (US)
Pages (from-to)166-178
Number of pages13
JournalGenes and Development
Volume22
Issue number2
DOIs
StatePublished - Jan 15 2008

Fingerprint

Basic Helix-Loop-Helix Transcription Factors
GABAergic Neurons
Spinal Cord
Transcription Factors
Notch Receptors
Neural Tube
Transducers
Cerebellum
Nervous System
Retina
Stem Cells
Neurons
Mutation
Proteins

Keywords

  • bHLH transcription factor
  • Cerebellum
  • Dorsal spinal cord
  • Neuronal specification
  • Notch
  • Ptf1a

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

A nonclassical bHLH-Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling. / Hori, Kei; Cholewa-Waclaw, Justyna; Nakada, Yuji; Glasgow, Stacey M.; Masui, Toshihiko; Henke, R. Michael; Wildner, Hendrik; Martarelli, Benedetta; Beres, Thomas M.; Epstein, Jonathan A.; Magnuson, Mark A.; MacDonald, Raymond J.; Birchmeier, Carmen; Johnson, Jane E.

In: Genes and Development, Vol. 22, No. 2, 15.01.2008, p. 166-178.

Research output: Contribution to journalArticle

Hori, K, Cholewa-Waclaw, J, Nakada, Y, Glasgow, SM, Masui, T, Henke, RM, Wildner, H, Martarelli, B, Beres, TM, Epstein, JA, Magnuson, MA, MacDonald, RJ, Birchmeier, C & Johnson, JE 2008, 'A nonclassical bHLH-Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling', Genes and Development, vol. 22, no. 2, pp. 166-178. https://doi.org/10.1101/gad.1628008
Hori, Kei ; Cholewa-Waclaw, Justyna ; Nakada, Yuji ; Glasgow, Stacey M. ; Masui, Toshihiko ; Henke, R. Michael ; Wildner, Hendrik ; Martarelli, Benedetta ; Beres, Thomas M. ; Epstein, Jonathan A. ; Magnuson, Mark A. ; MacDonald, Raymond J. ; Birchmeier, Carmen ; Johnson, Jane E. / A nonclassical bHLH-Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling. In: Genes and Development. 2008 ; Vol. 22, No. 2. pp. 166-178.
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