A novel 2-hour method for rapid preparation of permanent paraffin sections when treating melanoma in situ with mohs micrographic surgery

Raj Mallipeddi, Jeff Stark, Xian Jin Xie, Mark Matthews, R. Stan Taylor

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

BACKGROUND Distinguishing sun-induced melanocyte atypia from residual melanoma in situ (MIS) can be challenging, particularly when working with frozen sections. Immunostains such as melanoma-associated antigen recognized by T cells (MART-1) can assist, but paraffin sections provide an optimal means of analyzing melanocyte morphology. OBJECTIVE To verify the effectiveness of a 2-hour paraffin processing technique that uses microwave technology in the preparation of MIS sections. METHODS Twelve MIS debulk specimens were divided into 4 pieces with each piece processed 1 of 4 ways: our 2-hour paraffin technique with hematoxylin and eosin (H&E), conventional 24-hour paraffin processing with H&E, frozen sections with H&E, and frozen sections with MART-1 immunostaining. A Mohs surgeon and a dermatopathologist compared all specimens in a blinded fashion using a 3-point ranking scale to assess ease of visualizing normal melanocytes, ease of visualizing abnormal melanocytes, and overall ability to adequately visualize epidermal and dermal structures. RESULTS A nonparametric signed rank test indicated no significant differences between our microwave technique and conventional paraffin processing in all 3 criteria (p=.29,.63,.75, respectively). Our microwave technique was significantly better than frozen H&E sections for all 3 criteria (p=.046,.004,.005, respectively). CONCLUSION This rapid microwave tissue processing technique is comparable with conventional paraffin section processing. The authors have indicated no significant interest with commercial supporters.

Original languageEnglish (US)
Pages (from-to)1520-1526
Number of pages7
JournalDermatologic Surgery
Volume34
Issue number11
DOIs
StatePublished - Nov 2008

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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