A novel antioxidant gene from Mycobacterium tuberculosis

Sabine Ehrt, Michael U. Shiloh, Jia Ruan, Michael Choi, Stuart Gunzburg, Carl Nathan, Qiao Wen Xie, Lee W. Riley

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Among the major antimicrobial products of macrophages are reactive intermediates of the oxidation of nitrogen (RNI) and the reduction of oxygen (ROI). Selection of recombinants in acidified nitrite led to the cloning of a novel gene, noxR1, from a pathogenic clinical isolate of Mycobacterium tuberculosis. Expression of noxR1 conferred upon Escherichia coli and Mycobacterium smegmatis enhanced ability to resist RNI and ROI, whether the bacteria were exposed to exogenous compounds in medium or to endogenous products in macrophages. These studies provide the first identification of an RNI resistance mechanism in mycobacteria, point to a new mechanism for resistance to ROI, and raise the possibility that inhibition of the noxR1 pathway might enhance the ability of macrophages to control tuberculosis.

Original languageEnglish (US)
Pages (from-to)1885-1896
Number of pages12
JournalJournal of Experimental Medicine
Volume186
Issue number11
DOIs
StatePublished - Dec 1 1997

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ehrt, S., Shiloh, M. U., Ruan, J., Choi, M., Gunzburg, S., Nathan, C., Xie, Q. W., & Riley, L. W. (1997). A novel antioxidant gene from Mycobacterium tuberculosis. Journal of Experimental Medicine, 186(11), 1885-1896. https://doi.org/10.1084/jem.186.11.1885