A novel approach to produce biologically relevant chemical patterns at the nanometer scale: Selective molecular assembly patterning combined with colloidal lithography

Roger Michel, Ilya Reviakine, Duncan Sutherland, Christian Fokas, Gabor Csucs, Gaudenz Danuser, Nicholas D. Spencer, Marcus Textor

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

A novel patterning technique that combines colloidal patterning with selective adsorption of organic molecules has been used to chemically pattern metal oxide surfaces at length scale down to 50 nm. Lithographic nanofabrication using surface-assembled colloids as etch masks ("colloidal lithography") was used to create nanopillars of TiO2 (50-90 nm in diameter, ∼20 nm in height) on whole oxidized silicon or quartz wafer substrates. These nanopillars were then rendered hydrophobic by the selective self-assembly of an organophosphate, whereas a poly(ethylene glycol)-grafted copolymer Waas adsorbed onto the surrounding SiO2 rendering it protein resistant. This resulted in a two-component chemical pattern, displaying contrast with respect to protein adsorption (protein-adhesive pillars on nonadsorbing background). This property allows for efficient translation of the lithographic pattern into a surface protein pattern by two simple dip-and-rinse processes in aqueous solutions. The feasibility of the method and its quality were tested by adsorbing fluorescently labeled streptavidin and biotinylated phospholipid vesicles. The sequential adsorption steps were monitored by fluorescence microscopy, atomic force microscopy, and scanning near-field optical microscopy. These techniques conclusively demonstrated the utility of the described approach for chemical patterning surfaces on the nanometer scale over large areas.

Original languageEnglish (US)
Pages (from-to)8580-8586
Number of pages7
JournalLangmuir
Volume18
Issue number22
DOIs
StatePublished - Oct 29 2002

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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