A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5’-3’ Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe

James Franklin Tucker, Corina Ohle, Géza Schermann, Katja Bendrin, Wei Zhang, Tamás Fischer, Ke Zhang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including RNA interference (RNAi) and a 3’-5’ exoribonuclease complex, the exosome. Here, we report a new RNA-processing pathway that contributes to epigenetic gene silencing and assembly of heterochromatin mediated by 5’-3’ exoribonuclease Dhp1/Rat1/Xrn2. Dhp1 mutation causes defective gene silencing both at peri-centromeric regions and at the silent mating type locus. Intriguingly, mutation in either of the two well-characterized Dhp1-interacting proteins, the Din1 pyrophosphohydrolase or the Rhn1 transcription termination factor, does not result in silencing defects at the main heterochromatic regions. We demonstrate that Dhp1 interacts with heterochromatic factors and is essential in the sequential steps of establishing silencing in a manner independent of both RNAi and the exosome. Genomic and genetic analyses suggest that Dhp1 is involved in post-transcriptional silencing of repetitive regions through its RNA processing activity. The results describe the unexpected role of Dhp1/Rat1/Xrn2 in chromatin-based silencing and elucidate how various RNA-processing pathways, acting together or independently, contribute to epigenetic regulation of the eukaryotic genome.

Original languageEnglish (US)
Article numbere1005873
JournalPLoS Genetics
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

exoribonucleases
Schizosaccharomyces
Schizosaccharomyces pombe
Gene Silencing
gene silencing
Epigenomics
epigenetics
RNA
exosomes
Heterochromatin
heterochromatin
RNA Interference
RNA interference
Exosome Multienzyme Ribonuclease Complex
gene
Exosomes
mutation
Mutation
Nucleic Acid Repetitive Sequences
Eukaryota

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5’-3’ Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe. / Tucker, James Franklin; Ohle, Corina; Schermann, Géza; Bendrin, Katja; Zhang, Wei; Fischer, Tamás; Zhang, Ke.

In: PLoS Genetics, Vol. 12, No. 2, e1005873, 01.02.2016.

Research output: Contribution to journalArticle

Tucker, James Franklin ; Ohle, Corina ; Schermann, Géza ; Bendrin, Katja ; Zhang, Wei ; Fischer, Tamás ; Zhang, Ke. / A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5’-3’ Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe. In: PLoS Genetics. 2016 ; Vol. 12, No. 2.
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