A novel liver-directed gene delivery system using an autonomously replicating vector specifically expressed in AFP positive hepatoma cells

Ziying Yan, Jian Qiao, Huiyu Gong, Yunde Hou

Research output: Contribution to journalArticle

Abstract

A composite EBV-bssed expression vector, pEBAK, was constructed with the 5′-flanking sequence of human α-fetoprotein gene as a promoter. Completed to galactosylated histone, this vector with a foreign DNA insertion could be transferred into hepatic cells via the asialoglycoprotein receptor mediated endocytosis pathway. It was replicated as an episome, and its expression was restricted to the AFP positive hepatoma cells. This new gene delivery method has the following advantages: (i) absence of a potential toxicity is related to viral vectors; (ii) the targeting is specific to AFP positive hepatoma cells; (iii) the introduction of the EBV replicon into this system results in the high-level expression and long-term maintenance of the transferred gene. This novel gene delivery system has potential applications in gene therapy for the treatment of hepatocellular carcinoma.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalScience in China, Series C: Life Sciences
Volume41
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

Gene Transfer Techniques
hepatoma
gene transfer
Liver
Hepatocellular Carcinoma
Genes
Human Herpesvirus 4
liver
gene
Asialoglycoprotein Receptor
Fetal Proteins
replicon
Replicon
gene therapy
5' Flanking Region
endocytosis
cells
Endocytosis
histones
Gene therapy

Keywords

  • AFP promoter
  • EBV replicon vector
  • Hepatoma cell
  • Receptor-mediated gene transfer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

A novel liver-directed gene delivery system using an autonomously replicating vector specifically expressed in AFP positive hepatoma cells. / Yan, Ziying; Qiao, Jian; Gong, Huiyu; Hou, Yunde.

In: Science in China, Series C: Life Sciences, Vol. 41, No. 1, 01.01.1998, p. 80-86.

Research output: Contribution to journalArticle

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