A novel liver-directed gene delivery system using an autonomously replicating vector specifically expressed in AFP positive hepatoma cells

Ziying Yan, Jian Qiao, Huiyu Gong, Yunde Hou

Research output: Contribution to journalArticle

Abstract

A composite EBV-bssed expression vector, pEBAK, was constructed with the 5′-flanking sequence of human α-fetoprotein gene as a promoter. Completed to galactosylated histone, this vector with a foreign DNA insertion could be transferred into hepatic cells via the asialoglycoprotein receptor mediated endocytosis pathway. It was replicated as an episome, and its expression was restricted to the AFP positive hepatoma cells. This new gene delivery method has the following advantages: (i) absence of a potential toxicity is related to viral vectors; (ii) the targeting is specific to AFP positive hepatoma cells; (iii) the introduction of the EBV replicon into this system results in the high-level expression and long-term maintenance of the transferred gene. This novel gene delivery system has potential applications in gene therapy for the treatment of hepatocellular carcinoma.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalScience in China, Series C: Life Sciences
Volume41
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • AFP promoter
  • EBV replicon vector
  • Hepatoma cell
  • Receptor-mediated gene transfer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'A novel liver-directed gene delivery system using an autonomously replicating vector specifically expressed in AFP positive hepatoma cells'. Together they form a unique fingerprint.

  • Cite this