A novel mechanism of actin filament processive capping by formin: Solution of the rotation paradox

Tom Shemesh, Takanori Otomo, Michael K. Rosen, Alexander D. Bershadsky, Michael M. Kozlov

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The FH2 domains of formin family proteins act as processive cappers of actin filaments. Previously suggested stair-stepping mechanisms of processive capping imply that a formin cap rotates persistently in one direction with respect to the filament. This challenges the formin-mediated mechanism of intracellular cable formation. We suggest a novel scenario of processive capping that is driven by developing and relaxing torsion elastic stresses. Based on the recently discovered crystal structure of an FH2-actin complex, we propose a second mode of processive capping - the screw mode. Within the screw mode, the formin dimer rotates with respect to the actin filament in the direction opposite to that generated by the stair-stepping mode so that a combination of the two modes prevents persistent torsion strain accumulation. We determine an optimal regime of processive capping, whose essence is a periodic switch between the stair-stepping and screw modes. In this regime, elastic energy does not exceed feasible values, and supercoiling of actin filaments is prevented.

Original languageEnglish (US)
Pages (from-to)889-893
Number of pages5
JournalJournal of Cell Biology
Volume170
Issue number6
DOIs
StatePublished - Sep 12 2005

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Actin Cytoskeleton
Actins
Proteins
Direction compound

ASJC Scopus subject areas

  • Cell Biology

Cite this

A novel mechanism of actin filament processive capping by formin : Solution of the rotation paradox. / Shemesh, Tom; Otomo, Takanori; Rosen, Michael K.; Bershadsky, Alexander D.; Kozlov, Michael M.

In: Journal of Cell Biology, Vol. 170, No. 6, 12.09.2005, p. 889-893.

Research output: Contribution to journalArticle

Shemesh, Tom ; Otomo, Takanori ; Rosen, Michael K. ; Bershadsky, Alexander D. ; Kozlov, Michael M. / A novel mechanism of actin filament processive capping by formin : Solution of the rotation paradox. In: Journal of Cell Biology. 2005 ; Vol. 170, No. 6. pp. 889-893.
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