A novel mechanism of glucocorticoid-induced immune suppression: The inhibition of T cell-mediated terminal maturation of a murine dendritic cell line

Toshlyuki Kitajima, Kiyoshi Ariizumi, Paul R. Bergstresser, Akira Takashima

Research output: Contribution to journalArticle

78 Scopus citations


Working with the murine epidermal-derived dendritic cell (DC) line XS52, we have observed previously that antigen-specific interaction with T cells stimulates their 'terminal maturation' into fully professional DC. In this study we examined the impact of dexamethasone (DEX) on this T cell- induced event. When added to cocultures of XS52 DC and the KLH-specific Th1 clone HDK-1 in the presence of antigen, DEX at relatively low concentrations (10-9-10-7 M) prevented substantially or completely each of the changes that typify terminal maturation, including (a) secretion of relatively large amounts of IL-1β, IL-6, and TNFα; (b) loss of CD115 (colony-stimulating factor-1 receptor) expression and proliferative responsiveness to colony- stimulating factor-1; and (c) elevated expression of CD86 (B7-2). XS52 cells also underwent terminal maturation upon exposure to lipopolysaccharide alone, and DEX also inhibited effectively each of the same changes, indicating that DC can serve as the direct target of DEX. By contrast, DEX inhibited XS52 DC-stimulated IL-2 secretion by HDK-1 T cells, but not other changes that accompany T cell activation, including the secretion of IFNγ, and TNFα and the elevated expression of CD25, CD28, and CD44. These results reveal a new immunosuppressive mechanism of glucocorticoid action, that is, direct inhibition of T cell-mediated terminal maturation by DC.

Original languageEnglish (US)
Pages (from-to)142-147
Number of pages6
JournalJournal of Clinical Investigation
Issue number1
StatePublished - Jul 1 1996



  • CD115
  • CD28
  • CSF-1
  • dexamethasone
  • IL-1β

ASJC Scopus subject areas

  • Medicine(all)

Cite this